RHAMNOGALACTURONAN ACETYLESTERASE - A NOVEL ENZYME FROM ASPERGILLUS-ACULEATUS, SPECIFIC FOR THE DEACETYLATION OF HAIRY (RAMIFIED) REGIONS OF PECTINS

被引：51

作者：

SEARLEVANLEEUWEN, MJF ^{[1
]}

VANDENBROEK, LAM ^{[1
]}

SCHOLS, HA ^{[1
]}

BELDMAN, G ^{[1
]}

VORAGEN, AGJ ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR, BIOTECHNION, DEPT FOOD SCI, BOMENWEG 2, 6703 HD WAGENINGEN, NETHERLANDS

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 38卷 / 03期

关键词：

D O I：

10.1007/BF00170084

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhamnogalacturonan acetylesterase, able to specifically hydrolyse the acetyl esters present in modified hairy (ramified) regions (MHR) of apple pectin, was identified. The enzyme removed about 70% of the total acetyl groups in MHR. This acetylesterase did not cause the release of acetyl groups from a range of other acetylated substrates, either synthetic or extracted from plants, including the acetylated smooth regions present in beet pectin. Pretreatment of pectic polysaccharides in order to remove arabinose side chains had no effect on the acetyl release, nor was an effect found on the rate or degree of acetyl release, when the purified acetylesterase was combined with pectolytic enzymes, pectin methylesterase or arabinanases.

引用

页码：347 / 349

页数：3

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