MECHANISM OF SYNERGISTIC INDUCTION OF HEPATIC HEME OXYGENASE BY GLUTETHIMIDE AND IRON - STUDIES IN CULTURED CHICK-EMBRYO LIVER-CELLS

被引：31

作者：

CABLE, E ^{[1
]}

GREENE, Y ^{[1
]}

HEALEY, J ^{[1
]}

EVANS, CO ^{[1
]}

BONKOVSKY, H ^{[1
]}

机构：

[1] EMORY UNIV, DEPT MED, ATLANTA, GA 30322 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91690-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heme oxygenase, the rate controlling enzyme for heme catabolism, is inducible by a variety of treatments, some of which induce by a heme-dependent mechanism and others by a heme-independent mechanism. This work shows that, in cultured chick embryo liver cells, synergistic induction of heme oxygenase by iron, added with the phenobarbital-like drug, glutethimide was heme-dependent. Addition of an inhibitor of heme biosynthesis abolished the synergistic induction of heme oxygenase providing evidence for the heme-dependent mechanism of induction. Glutethimide and iron appeared to induce at the transcriptional level since both heme oxygenase mRNA and protein levels correlate with changes in heme oxygenase activity. © 1990.

引用

页码：176 / 181

页数：6

共 25 条

[21] HORMONAL REQUIREMENTS FOR THE INDUCTION OF CYTOCHROME-P450 IN HEPATOCYTES CULTURED IN A SERUM-FREE MEDIUM [J].