STUDIES OF NONCOVALENT INTERACTIONS OF ACTINOMYCIN-D WITH SINGLE-STRANDED OLIGODEOXYNUCLEOTIDES BY ION-SPRAY MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY
被引:57
作者:
HSIEH, YL
论文数: 0引用数: 0
h-index: 0
机构:CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
HSIEH, YL
LI, YT
论文数: 0引用数: 0
h-index: 0
机构:CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
LI, YT
HENION, JD
论文数: 0引用数: 0
h-index: 0
机构:CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
HENION, JD
GANEM, B
论文数: 0引用数: 0
h-index: 0
机构:CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
GANEM, B
机构:
[1] CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucleotides were investigated as a function of pH. Non-covalent AMD/ssDNA complexes disappeared at low pH, and no ssDNA complexes were detected with rifampicin (RP). Moreover, the different binding affinities between AMD and ssDNA with and without (5')G-C-3' Or C-5'-G(3') base sequences were also demonstrated using ion spray mass spectrometry. Additional support for the non-covalent nature of binding in AMD/ssDNA complexes was obtained from tandem mass spectrometry.