STUDIES OF NONCOVALENT INTERACTIONS OF ACTINOMYCIN-D WITH SINGLE-STRANDED OLIGODEOXYNUCLEOTIDES BY ION-SPRAY MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY

被引:57
作者
HSIEH, YL
LI, YT
HENION, JD
GANEM, B
机构
[1] CORNELL UNIV,NEW YORK STATE COLL VET MED,DIAGNOST LAB,ITHACA,NY 14850
[2] CORNELL UNIV,BAKER LAB,DEPT CHEM,ITHACA,NY 14853
关键词
D O I
10.1002/bms.1200230506
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucleotides were investigated as a function of pH. Non-covalent AMD/ssDNA complexes disappeared at low pH, and no ssDNA complexes were detected with rifampicin (RP). Moreover, the different binding affinities between AMD and ssDNA with and without (5')G-C-3' Or C-5'-G(3') base sequences were also demonstrated using ion spray mass spectrometry. Additional support for the non-covalent nature of binding in AMD/ssDNA complexes was obtained from tandem mass spectrometry.
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页码:272 / 276
页数:5
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