PREPARATION OF CATHEPSIN-B AND CATHEPSIN-H BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF THEIR CATALYTIC SITES BY REACTION WITH A THIOL-SPECIFIC 2-PROTONIC-STATE REACTIVITY PROBE - KINETIC-STUDY OF CATHEPSIN-B AND CATHEPSIN-H EXTENDING INTO ALKALINE MEDIA AND A RAPID SPECTROSCOPIC TITRATION OF CATHEPSIN-H AT PH3-4

被引：42

作者：

WILLENBROCK, F

BROCKLEHURST, K

机构：

来源：

BIOCHEMICAL JOURNAL | 1985年 / 227卷 / 02期

关键词：

D O I：

10.1042/bj2270511

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：511 / 519

页数：9

共 30 条

[1] PREPARATION OF MODIFIED AGAROSE GELS CONTAINING THIOL-GROUPS [J].

AXEN, R ;

DREVIN, H ;

CARLSSON, J .

ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1975, 29 (04) :471-474

[2]

BAJKOWSKI AS, 1983, J BIOL CHEM, V258, P1645

[3]

Barrett A. J., 1980, ENZYME REGULATION ME, P307

[4] NEW ASSAY FOR CATHEPSIN B1 AND OTHER THIOL PROTEINASES [J].

BARRETT, AJ .

ANALYTICAL BIOCHEMISTRY, 1972, 47 (01) :280-&

[5] HUMAN CATHEPSIN-B1 - PURIFICATION AND SOME PROPERTIES OF ENZYME [J].

BARRETT, AJ .

BIOCHEMICAL JOURNAL, 1973, 131 (04) :809-+

[6]

BARRETT AJ, 1981, ACTA BIOL MED GER, V40, P1513

[7]

BARRETT AJ, 1981, METHOD ENZYMOL, V80, P535

[8] DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES [J].

BROCKLEHURST, K ;

BAINES, BS ;

MALTHOUSE, JPG .

BIOCHEMICAL JOURNAL, 1981, 197 (03) :739-746

[9] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX [J].

BROCKLEHURST, K ;

CARLSSON, J ;

KIERSTAN, MP ;

CROOK, EM .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :573-584

[10] DIFFERENCES BETWEEN THE ELECTRIC-FIELDS OF THE CATALYTIC SITES OF PAPAIN AND ACTINIDIN DETECTED BY USING THE THIOL-LOCATED NITROBENZOFURAZAN LABEL AS A SPECTROSCOPIC REPORTER GROUP [J].

BROCKLEHURST, K ;

SALIH, E ;

LODWIG, TS .

BIOCHEMICAL JOURNAL, 1984, 220 (02) :609-612

← 1 2 3 →