EFFECT OF BUTYLATED HYDROXYANISOLE ON CONIDIAL GERMINATION OF TOXIGENIC SPECIES OF ASPERGILLUS-FLAVUS AND ASPERGILLUS-PARASITICUS

The fungitoxic effect of butylated hydroxyanisole (BHA) on germination of conidia from seven toxigenic strains of Aspergillus was examined on potato dextrose agar (PDA). At concentrations of 200-mu-g/ml and above, BHA prevented germination of conidia in all of the test fungi after 24 and 48 h of incubation. On the other hand, at 100-mu-g/ml BHA reduced the rate of germination. When an impregnated disk assay was employed, distinct inhibition zones were observed at 200, 400, 800, and 1000-mu-g/disk of BHA after 24 h of incubation. After 48 h of incubation, distinct inhibition zones were still observed at 800 and 1000-mu-g/disk.