IMPROVED METHOD FOR IDENTIFICATION AND DETERMINATION OF EPSILON-(GAMMA-GLUTAMYL)LYSINE CROSS-LINK IN PROTEIN USING PROTEOLYTIC DIGESTION AND DERIVATIZATION WITH PHENYL ISOTHIOCYANATE FOLLOWED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION

被引：72

作者：

SATO, K

TSUKAMASA, Y

IMAI, C

OHTSUKI, K

SHIMIZU, Y

KAWABATA, M

机构：

[1] MARUDAI FOOD CO LTD,CENT RES INST,TAKATSUKI,OSAKA 569,JAPAN

[2] KOBE GAKUIN WOMENS JR COLL,KOBE 653,JAPAN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1992年 / 40卷 / 05期

关键词：

D O I：

10.1021/jf00017a019

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An improved method for the identification and determination of epsilon-(gamma-glutamyl)lysine cross-link in food protein is described. The cross-link was liberated by exhaustive proteolytic digestion and derivatized with PITC. The derivatives were resolved by HPLC. The present method enables one to determine simultaneously protein-constituting amino acids and epsilon-(gamma-glutamyl)lysine and thereby facilitates the monitoring of progress of proteolytic digestion. The PTC-epsilon-(gamma-glutamyl)lysine peak was collected and confirmed by conversion to a stable PTH form followed by HCl hydrolysis. The present method was successfully applied for the identification and determination of the cross-link in sardine meat gel.

引用

页码：806 / 810

页数：5

共 21 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF EPSILON-(GAMMA-GLUTAMYL)LYSINE AND MONO-GAMMA-GLUTAMYL AND BIS-GAMMA-GLUTAMYL DERIVATIVES OF PUTRESCINE AND SPERMIDINE [J].

BENINATI, S ;

MARTINET, N ;

FOLK, JE .

JOURNAL OF CHROMATOGRAPHY, 1988, 443 :329-335

[2]

BIDLINGMEYER BA, 1984, J CHROMATOGR, V336, P105

[3]

BRUCKNER H, 1987, J CHROMATOGR, V386, P251, DOI 10.1016/S0021-9673(01)94602-3

[4]

FESUS L, 1989, FEBS LETT, V245, P155

[5]

FOLK JE, 1973, ADV ENZYMOL RAMB, V38, P109

[6] HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC PROCEDURE FOR THE DETERMINATION OF EPSILON-(GAMMA-GLUTAMYL)LYSINE IN PROTEINS [J].

GRIFFIN, M ;

WILSON, J ;

LORAND, L .

ANALYTICAL BIOCHEMISTRY, 1982, 124 (02) :406-413

[7] INCORPORATION OF AMINO-ACIDS INTO FOOD PROTEINS BY TRANSGLUTAMINASE [J].

IKURA, K ;

YOSHIKAWA, M ;

SASAKI, R ;

CHIBA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (11) :2587-2592

[8] CROSSLINKING OF CASEIN COMPONENTS BY TRANSGLUTAMINASE [J].

IKURA, K ;

KOMETANI, T ;

YOSHIKAWA, M ;

SASAKI, R ;

CHIBA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (07) :1567-1573

[9] CROSSLINKING OF SOYBEAN-7S AND SOYBEAN-11S PROTEINS BY TRANSGLUTAMINASE [J].

IKURA, K ;

KOMETANI, T ;

SASAKI, R ;

CHIBA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (12) :2979-2984

[10] OVOMUCIN FOOD PROTEIN CONJUGATES PREPARED THROUGH THE TRANSGLUTAMINASE REACTION [J].

KATO, A ;

WADA, T ;

KOBAYASHI, K ;

SEGURO, K ;

MOTOKI, M .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (04) :1027-1031

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