A YAC CONTIG MAP OF PLASMODIUM-FALCIPARUM CHROMOSOME-4 - CHARACTERIZATION OF A DNA AMPLIFICATION BETWEEN 2 RECENTLY SEPARATED ISOLATES

被引：22

作者：

RUBIO, JP

TRIGLIA, T

KEMP, DJ

DEBRUIN, D

RAVETCH, JV

COWMAN, AF

机构：

[1] WALTER & ELIZA HALL INST MED RES,MELBOURNE,VIC 3050,AUSTRALIA

[2] MENZIES SCH HLTH RES,DARWIN,NT,AUSTRALIA

[3] MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021

来源：

GENOMICS | 1995年 / 26卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1016/0888-7543(95)80200-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have generated a physical map of Plasmodium falciparum chromosome 4 using yeast artificial chromosomes (YACs). The map is defined by a YAC contig spanning approximately 1.05 Mb, which has been restriction mapped to a resolution of 30 kb and is punctuated by 22 sequence tagged sites. The physical information obtained has enabled us to compare and contrast the structure of chromosome 4 in detail between FCR3 and B8, two recently separated isolates of P. falciparum, leading to characterization of a novel chromosome polymorphism occurring in a subtelomeric region. Comparison of chromosomes 4 from 10 different isolates has shown that chromosome size polymorphisms are restricted to both subtelomeric regions. These analyses provide a high-resolution physical map that will be important to complement genetic analysis of this human pathogen. (C) 1995 Academic Press, Inc.

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页码：192 / 198

页数：7

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