OPTIMIZATION OF LIQUID-CHROMATOGRAPHIC PARAMETERS FOR THE SEPARATION OF PRIORITY PHENOLS BY USING MIXED-LEVEL ORTHOGONAL ARRAY DESIGN

被引：25

作者：

CHEE, KK ^{[1
]}

LAN, WG ^{[1
]}

WONG, MK ^{[1
]}

LEE, HK ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,DEPT CHEM,SINGAPORE 0511,SINGAPORE

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 312卷 / 03期

关键词：

LIQUID CHROMATOGRAPHY; MIXED-LEVEL ORTHOGONAL ARRAY DESIGN; CHEMOMETRICS; SUBSTITUTED PHENOLS; PHENOLS;

D O I：

10.1016/0003-2670(95)00214-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mixed-level orthogonal array design (OAD) as a chemometric method has been employed to optimize liquid chromatographic (LC) conditions far the separation of environmental pollutants. Six parameters were examined by OAD, namely reversed-phase (C-18) LC columns from different manufacturers, content of methanol and acetonitrile in the mobile phase, initial time-duration of a fixed mobile phase composition, time-duration of a gradient elution programme, and mobile phase flow rate. The optimization of these parameters for the determination of eleven priority substituted phenols was carried out to demonstrate the applicability of mixed-level OAD in environmental analytical chemistry in which the use of trial-and-error procedures is often unsatisfactory due to sample complexity. The advantages and the disadvantages of mixed-level OAD were discussed.

引用

页码：271 / 280

页数：10

共 45 条

[1] TRACE DETERMINATION OF PHENOLIC-COMPOUNDS IN WATER BY REVERSED PHASE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION USING A CARBON-POLYETHYLENE TUBULAR ANODE [J].