SYNTHESIS AND CHARACTERIZATION OF A SUBSTRATE FOR T4 ENDONUCLEASE-V CONTAINING A PHOSPHORODITHIOATE LINKAGE AT THE THYMINE DIMER SITE

被引：87

作者：

MURATA, T ^{[1
]}

IWAI, S ^{[1
]}

OHTSUKA, E ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 24期

关键词：

D O I：

10.1093/nar/18.24.7279

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A dodecadeoxyribonucleotide containing a cis-syn thymine dimer with a phosphorodithioate linkage was synthesized on a solid support using a dinucleotide coupling unit prepared by UV-irradiation of dithymidine monophosphorodithioate followed by S- and 5'-O-protection and 3'-phosphitylation. A photodimer-containing dodecamer without phosphate modification was also synthesized. The dodecamers were hybridized to the complimentary sequence, and the duplexes used as substrates for T4 endonuclease V. This enzyme cleaved the phosphate-modified substrate more slowly than the unmodified duplex with the same dissociation constant.

引用

页码：7279 / 7286

页数：8

共 22 条

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