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NEGATIVE AND POSITIVE ELEMENTS IN THE PROMOTER REGION OF THE HUMAN APOFERRITIN L-GENE

被引：5
作者：
DAGOSTINO, P
FANIELLO, MC
QUARESIMA, B
BEVILACQUA, MA
TIANO, MT
AMMENDOLA, R
CIMINO, F
COSTANZO, F
机构：
[1] UNIV NAPLES FEDERICO II,DIPARTIMENTO BIOCHIM & BIOTECNOL MED,I-80131 NAPLES,ITALY
[2] UNIV REGGIO CALABRIA,DIPARTIMENTO MED SPERIMENTALE & CLIN,I-88100 CATANZARO,ITALY
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.2470
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have characterized the promoter of the human gene coding for the apoferritin L subunit. Transient transfections of 5' and 3' deletion mutants indicate that the efficiency of the L promoter depends on both negative and positive cis-elements, located upstream and downstream of the transcription start point. DNasel footprinting analysis of this DNA region revealed the presence of five protected segments. The most upstream one (element 1) corresponds to the negative cis-element and is recognized by factor(s) sharing a GC-sequence specificity. Three positive elements are in the region upstream of the start of transcription; a fifth positive cis-element (element 5) is localized in the first exon of the L gene. (C) 1995 Academic Press, Inc.
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页码：329 / 337
页数：9
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