CONDITIONAL REGULATORY ELEMENTS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 LONG TERMINAL REPEAT

被引:4
作者
ARYA, SK
MOHR, JR
机构
[1] Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda
关键词
D O I
10.1099/0022-1317-75-9-2253
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the U3 region, located upstream of the enhancer-promoter region. These elements acted in a cell type-specific manner, being most active in human lymphocytic CEM cells, more active in Jurkat cells than in human monocytic U937 cells and least active in epithelioid HeLa cells. The down-modulatory effect of the negative regulatory element was abolished by HIV-2 Tat, suggesting the involvement of upstream DNA elements in optimal Tat-mediated trans-activation. The sequence elements that respond to T cell activation signals were also located in the upstream U3 region. Notably, the magnitude of the effect of the upstream regulatory elements depended on the basal activity of the LTR, which was also cell type-dependent. This emphasizes the importance of the cell-specific transcriptional factors and other effecters in regulating HIV gene expression. These observations may be relevant to the cell type-specific restriction of virus replication in vivo.
引用
收藏
页码:2253 / 2260
页数:8
相关论文
共 37 条
[31]   CONTRIBUTION OF THE TATA MOTIF TO TAT-MEDIATED TRANSCRIPTIONAL ACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS GENE-EXPRESSION [J].
OLSEN, HS ;
ROSEN, CA .
JOURNAL OF VIROLOGY, 1992, 66 (09) :5594-5597
[32]   HIV-INFECTION IS ACTIVE AND PROGRESSIVE IN LYMPHOID-TISSUE DURING THE CLINICALLY LATENT STAGE OF DISEASE [J].
PANTALEO, G ;
GRAZIOSI, C ;
DEMAREST, JF ;
BUTINI, L ;
MONTRONI, M ;
FOX, CH ;
ORENSTEIN, JM ;
KOTLER, DP ;
FAUCI, AS .
NATURE, 1993, 362 (6418) :355-358
[33]   PREVALENCE OF AND MORTALITY FROM HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 IN BISSAU, WEST-AFRICA [J].
POULSEN, AG ;
AABY, P ;
FREDERIKSEN, K ;
LAURITZEN, E ;
KVINESDAL, B ;
MOLBAK, K ;
DIAS, F .
LANCET, 1989, 1 (8642) :827-831
[34]   THE LOCATION OF CIS-ACTING REGULATORY SEQUENCES IN THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-III (HTLV-III/LAV) LONG TERMINAL REPEAT [J].
ROSEN, CA ;
SODROSKI, JG ;
HASELTINE, WA .
CELL, 1985, 41 (03) :813-823
[35]   HUMAN-IMMUNODEFICIENCY-VIRUS LONG TERMINAL REPEAT RESPONDS TO T-CELL ACTIVATION SIGNALS [J].
TONGSTARKSEN, SE ;
LUCIW, PA ;
PETERLIN, BM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6845-6849
[36]  
TONGSTARKSEN SE, 1990, J IMMUNOL, V145, P4348
[37]   LONG-TERM CULTURES OF HTLV-III INFECTED T-CELLS - A MODEL OF CYTOPATHOLOGY OF T-CELL DEPLETION IN AIDS [J].
ZAGURY, D ;
BERNARD, J ;
LEONARD, R ;
CHEYNIER, R ;
FELDMAN, M ;
SARIN, PS ;
GALLO, RC .
SCIENCE, 1986, 231 (4740) :850-853