学术探索
学术期刊
新闻热点
数据分析
智能评审

OXYGEN-REGULATED IN-VITRO TRANSCRIPTION OF RHIZOBIUM-MELILOTI NIFA AND FIXK GENES

被引：72
作者：
REYRAT, JM
DAVID, M
BLONSKI, C
BOISTARD, P
BATUT, J
机构：
[1] INRA,CNRS,BIOL MOLEC RELAT PLANTES MICROORGANISMES LAB,BP 27,F-31326 CASTANET TOLOSAN,FRANCE
[2] UNIV TOULOUSE 3,CHIM ORGAN BIOL GRP,CNRS,URA 470,F-31062 TOULOUSE,FRANCE
来源：
JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 21期
关键词：
D O I：
10.1128/jb.175.21.6867-6872.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Oxygen concentration regulates the expression of nitrogen fixation genes in the symbiotic bacterium Rhizobium meliloti. We demonstrate that two proteins, FixL and FixJ, that belong to the two-component family of regulatory proteins are necessary and sufficient for oxygen-regulated in vitro transcription of the two key regulatory genes, nifA and fixK. We show directly that FixJ is a transcriptional activator, working in conjunction with the RNA polymerase sigma 70 holoenzyme. Addition of FixL122, a soluble form of the sensor FixL protein, to the transcription assay enhanced FixJ transcriptional activity in response to low oxygen concentration. This enhancement of FixJ activity was correlated with FixJ phosphorylation.
引用
收藏
页码：6867 / 6872
页数：6
相关论文
共 32 条
[1]   OXYGEN REGULATION OF NIFA TRANSCRIPTION INVITRO [J].
AGRON, PG ;
DITTA, GS ;
HELINSKI, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) :3506-3510
[2]   PROKARYOTIC SIGNAL TRANSDUCTION MEDIATED BY SENSOR AND REGULATOR PROTEIN PAIRS [J].
ALBRIGHT, LM ;
HUALA, E ;
AUSUBEL, FM .
ANNUAL REVIEW OF GENETICS, 1989, 23 :311-336
[3]   INVITRO ACTIVITY OF THE NITROGEN-FIXATION REGULATORY PROTEIN FIXJ FROM RHIZOBIUM-MELILOTI [J].
BATUT, J ;
SANTERO, E ;
KUSTU, S .
JOURNAL OF BACTERIOLOGY, 1991, 173 (18) :5914-5917
[4]   FIXK, A GENE HOMOLOGOUS WITH FNR AND CRP FROM ESCHERICHIA-COLI, REGULATES NITROGEN-FIXATION GENES BOTH POSITIVELY AND NEGATIVELY IN RHIZOBIUM-MELILOTI [J].
BATUT, J ;
DAVERANMINGOT, ML ;
JACOBS, MDJ ;
GARNERONE, AM ;
KAHN, D .
EMBO JOURNAL, 1989, 8 (04) :1279-1286
[5]  
BATUT J, 1992, ADV MOL GENETICS PLA, V2, P183
[6]   GENE-REGULATION BY STEROID-HORMONES [J].
BEATO, M .
CELL, 1989, 56 (03) :335-344
[7]   SIGNAL TRANSDUCTION PATHWAYS INVOLVING PROTEIN-PHOSPHORYLATION IN PROKARYOTES [J].
BOURRET, RB ;
BORKOVICH, KA ;
SIMON, MI .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :401-441
[8]   MOLECULAR SIGNALS IN THE INTERACTIONS BETWEEN PLANTS AND MICROBES [J].
CLARKE, HRG ;
LEIGH, JA ;
DOUGLAS, CJ .
CELL, 1992, 71 (02) :191-199
[9]   PROTEIN-PHOSPHORYLATION IN PROKARYOTES [J].
COZZONE, AJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1988, 42 :97-125
[10]   CASCADE REGULATION OF NIF GENE-EXPRESSION IN RHIZOBIUM-MELILOTI [J].
DAVID, M ;
DAVERAN, ML ;
BATUT, J ;
DEDIEU, A ;
DOMERGUE, O ;
GHAI, J ;
HERTIG, C ;
BOISTARD, P ;
KAHN, D .
CELL, 1988, 54 (05) :671-683
1234