COMPLEMENTATION OF AN ESCHERICHIA-COLI GLYCOLYSIS MUTANT BY GIARDIA-LAMBLIA TRIOSEPHOSPHATE ISOMERASE

被引：41

作者：

MOWATT, MR

WEINBACH, EC

HOWARD, TC

NASH, TE

机构：

[1] Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, Building 4

来源：

EXPERIMENTAL PARASITOLOGY | 1994年 / 78卷 / 01期

关键词：

TIM; TPI; EC; 5.3.1.1; PCR; PRIMER EXTENSION; GENE EXPRESSION;

D O I：

10.1006/expr.1994.1008

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The Giardia lamblia triosephosphate isomerase (TIM) gene was cloned using a probe generated by a polymerase chain reaction that employed primers complementary to highly conserved regions of TIM. The nucleotide sequence predicts a protein that is 38 and 47% identical to TIM from prokaryotic and eukaryotic sources, respectively. Like all other Giardia protein-coding genes studied thus far, the TIM gene lacks introns and is transcribed to yield a polyadenylated mRNA with an extremely short 5' untranslated region. The Giardia TIM gene complemented an Escherichia coli triosephosphate isomerase deletion mutant. The simplicity and success of complementation suggests its general utility in cloning Giardia genes of known function.© 1994 Academic press Inc. © 1994 Academic press, Inc.

引用

页码：85 / 92

页数：8

共 35 条

[21] OCCURRENCE OF PYROPHOSPHATE - FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE IN GIARDIA-LAMBLIA TROPHOZOITES [J].

MERTENS, E .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 40 (01) :147-149

[22]

Miller J.H., 1972, EXPT MOL GENETICS, P1

[23] CARBOXY-TERMINAL SEQUENCE CONSERVATION AMONG VARIANT-SPECIFIC SURFACE-PROTEINS OF GIARDIA-LAMBLIA [J].

MOWATT, MR ;

AGGARWAL, A ;

NASH, TE .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (02) :215-228

[24]

MURTAGH JJ, 1992, J BIOL CHEM, V267, P9654

[25]

NASH TE, 1988, J IMMUNOL, V141, P636

[26] IDENTIFICATION AND CHARACTERIZATION OF A GIARDIA-LAMBLIA GROUP-SPECIFIC GENE [J].

NASH, TE ;

MOWATT, MR .

EXPERIMENTAL PARASITOLOGY, 1992, 75 (04) :369-378

[27] HUMAN URACIL-DNA GLYCOSYLASE COMPLEMENTS ESCHERICHIA-COLI UNG MUTANTS [J].

OLSEN, LC ;

AASLAND, R ;

KROKAN, HE ;

HELLAND, DE .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4473-4478

[28] 3' NON-CODING REGION SEQUENCES IN EUKARYOTIC MESSENGER-RNA [J].

PROUDFOOT, NJ ;

BROWNLEE, GG .

NATURE, 1976, 263 (5574) :211-214

[29] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[30] THE PATHWAY OF ARGININE CATABOLISM IN GIARDIA-INTESTINALIS [J].

SCHOFIELD, PJ ;

EDWARDS, MR ;

MATTHEWS, J ;

WILSON, JR .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (01) :29-36

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