STABLE EXPRESSION OF THE NEURONAL BI (CLASS-A) CALCIUM-CHANNEL IN BABY HAMSTER-KIDNEY CELLS

被引：35

作者：

NIIDOME, T ^{[1
]}

TERAMOTO, T ^{[1
]}

MURATA, Y ^{[1
]}

TANAKA, I ^{[1
]}

SETO, T ^{[1
]}

SAWADA, K ^{[1
]}

MORI, Y ^{[1
]}

KATAYAMA, K ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT MOLEC PHARMACOL & BIOPHYS,CINCINNATI,OH 45267

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2399

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmids containing the BI (alpha(1)) cDNA with the dihydrofolate reductase (DHFR) gene, skeletal muscle alpha(2)-subunit cDNA with the neo marker gene, and beta-subunit cDNA were co-transfected into baby hamster kidney (BHK) cells. BHK cells lack endogenous calcium channel activity. Twenty percent of the methotrexate (MTX) and G418 resistant clones were found to express calcium channel activity using the patch-clamp technique. A single clone,BHKBI147, demonstrated stable electrophysiological characteristics over 20 passages. Ca2+ currents of the BI channel in BHKBL147 cells were largely blocked by a specific P-type blocker, omega-AgaIVA, with an IC50 of 150 nM. Unlike the BI channel, Ca2+ currents of cardiac L-type channels expressed in BHK cells were completely blocked by the L-type antagonist, nifedipine, with an IC50 of 56 nM. (C) 1994 Academic Press, Inc.

引用

页码：1821 / 1827

页数：7

共 20 条

[1] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE OMEGA-CONOTOXIN-SENSITIVE N-TYPE CALCIUM-CHANNEL FROM RABBIT BRAIN [J].

FUJITA, Y ;

MYNLIEFF, M ;

DIRKSEN, RT ;

KIM, MS ;

NIIDOME, T ;

NAKAI, J ;

FRIEDRICH, T ;

IWABE, N ;

MIYATA, T ;

FURUICHI, T ;

FURUTAMA, D ;

MIKOSHIBA, K ;

MORI, Y ;

BEAM, KG .

NEURON, 1993, 10 (04) :585-598

[2] SELECTIVE COUPLING WITH K+ CURRENTS OF MUSCARINIC ACETYLCHOLINE-RECEPTOR SUBTYPES IN NG108-15 CELLS [J].

FUKUDA, K ;

HIGASHIDA, H ;

KUBO, T ;

MAEDA, A ;

AKIBA, I ;

BUJO, H ;

MISHINA, M ;

NUMA, S .

NATURE, 1988, 335 (6188) :355-358

[3] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[4]

HESS P, 1990, ANNU REV NEUROSCI, V13, P337, DOI 10.1146/annurev.neuro.13.1.337

[5] CONSTRUCTION OF A MODULAR DIHYDROFOLATE-REDUCTASE CDNA GENE - ANALYSIS OF SIGNALS UTILIZED FOR EFFICIENT EXPRESSION [J].

KAUFMAN, RJ ;

SHARP, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (11) :1304-1319

[6] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE CARDIAC DIHYDROPYRIDINE-SENSITIVE CALCIUM-CHANNEL [J].

MIKAMI, A ;

IMOTO, K ;

TANABE, T ;

NIIDOME, T ;

MORI, Y ;

TAKESHIMA, H ;

NARUMIYA, S ;

NUMA, S .

NATURE, 1989, 340 (6230) :230-233

[7] MOLECULAR DISTINCTION BETWEEN FETAL AND ADULT FORMS OF MUSCLE ACETYLCHOLINE-RECEPTOR [J].

MISHINA, M ;

TAKAI, T ;

IMOTO, K ;

NODA, M ;

TAKAHASHI, T ;

NUMA, S ;

METHFESSEL, C ;

SAKMANN, B .

NATURE, 1986, 321 (6068) :406-411

[8] EXPRESSION OF FUNCTIONAL ACETYLCHOLINE-RECEPTOR FROM CLONED CDNAS [J].

MISHINA, M ;

KUROSAKI, T ;

TOBIMATSU, T ;

MORIMOTO, Y ;

NODA, M ;

YAMAMOTO, T ;

TERAO, M ;

LINDSTROM, J ;

TAKAHASHI, T ;

KUNO, M ;

NUMA, S .

NATURE, 1984, 307 (5952) :604-608

[9] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION FROM COMPLEMENTARY-DNA OF A BRAIN CALCIUM-CHANNEL [J].

MORI, Y ;

FRIEDRICH, T ;

KIM, MS ;

MIKAMI, A ;

NAKAI, J ;

RUTH, P ;

BOSSE, E ;

HOFMANN, F ;

FLOCKERZI, V ;

FURUICHI, T ;

MIKOSHIBA, K ;

IMOTO, K ;

TANABE, T ;

NUMA, S .

NATURE, 1991, 350 (6317) :398-402

[10] MOLECULAR-CLONING AND CHARACTERIZATION OF A NOVEL CALCIUM-CHANNEL FROM RABBIT BRAIN [J].

NIIDOME, T ;

KIM, MS ;

FRIEDRICH, T ;

MORI, Y .

FEBS LETTERS, 1992, 308 (01) :7-13

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