CYTOCHROME-P450 IN RAT ASTROCYTES IN-VIVO AND IN-VITRO - INTRACELLULAR-LOCALIZATION AND INDUCTION BY PHENYTOIN

被引:29
作者
KEMPERMANN, G
KNOTH, R
GEBICKEHAERTER, PJ
STOLZ, BJ
VOLK, B
机构
[1] UNIV FREIBURG, DEPT NEUROPATHOL, D-79104 FREIBURG, GERMANY
[2] UNIV FREIBURG, DEPT PSYCHIAT, D-79104 FREIBURG, GERMANY
关键词
ASTROCYTES; CELL CULTURES; ELECTRON MICROSCOPY;
D O I
10.1002/jnr.490390509
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Cytochrome P450IIB1,2 (nomenclature according to Nelson et al., DNA Cell Biol 12:1-51, 1993 and Volk et al., Neuroscience 42:215-235, 1991) immunoreactivity (P450-LR) is associated with astrocytes both in vivo and in vitro, Although they are unevenly distributed throughout the brain with a preference for phylogenetically elder parts, no significant differences between astrocytes prepared from different brain regions were observed in astrocyte cultures, The percentage of strongly immunoreactive astrocytes decreased from 40% after 7 days in culture to 15% after 21 days, Essentially all astrocytes have a low but significant P450-IR within this interval. Preembedding immunoelectron microscopy revealed peroxidase reaction products on the endoplasmic reticulum and on the outer membranes of mitochondrial and nuclear envelopes, Phenytoin (1 mu M) added to the medium for 7 days significantly (1.22-fold) increased the amount of total P450 in astrocyte homogenates as measured by spectrophotometry. Considerably more immunoreactive cells (1.5-fold) were found in treated cultures than in controls. (C) 1991 Wiley-Liss, Inc.
引用
收藏
页码:576 / 588
页数:13
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