DNA PROBES FOR THE Y-CHROMOSOME OF SILENE-LATIFOLIA, A DIOECIOUS ANGIOSPERM

被引:69
作者
MULCAHY, DL
WEEDEN, NF
KESSELI, R
CARROLL, SB
机构
[1] CORNELL UNIV,DEPT HORT SCI,GENEVA,NY 14456
[2] UNIV CALIF DAVIS,DEPT VEGETABLE CROPS,DAVIS,CA 95616
[3] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47405
来源
SEXUAL PLANT REPRODUCTION | 1992年 / 5卷 / 01期
关键词
SEX CHROMOSOME MARKERS; Y-CHROMOSOME; ANGIOSPERMS; SILENE-LATIFOLIA; MELANDRIUM-ALBUM;
D O I
10.1007/BF00714562
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In order to obtain markers for the Y chromosome of Silene latifolia, we pooled equal weights of leaf tissue from 18 female siblings into one sample and repeated the process with 18 male siblings. Pooling was intended to provide a common genetic background for each sample, leaving the absence or presence of the Y chromosome as the primary difference between the two samples. DNA was extracted from each sample and subjected to polymerase chain reaction (PCR) amplification with arbitrary 10 bp primers. Four of 60 primers used gave an amplification with the male DNA not found among those from the female DNA. Each of these was subsequently shown to provide a reliable marker for the Y chromosome.
引用
收藏
页码:86 / 88
页数:3
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