INVIVO FUNCTION OF THE PROTEASOME IN THE UBIQUITIN PATHWAY

被引：138

作者：

SEUFERT, W ^{[1
]}

JENTSCH, S ^{[1
]}

机构：

[1] MAX PLANCK GESELL,FRIEDRICH MIESCHER LAB,SPEMANNSTR 37-39,W-7400 TUBINGEN,GERMANY

来源：

EMBO JOURNAL | 1992年 / 11卷 / 08期

关键词：

PROTEASOME; PROTEIN DEGRADATION; UBIQUITIN SYSTEM; YEAST;

D O I：

10.1002/j.1460-2075.1992.tb05379.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A major eukaryotic proteolytic system is known to require the covalent attachment of ubiquitin to substrates prior to their degradation, yet the proteinase involved remains poorly defined. The proteasome, a large conserved multi-subunit protein complex of the cytosol and the nucleus, has been implicated in a variety of cellular functions. It is shown here that a yeast mutant with a defective proteasome fails to degrade proteins which are subject to ubiquitin-dependent proteolysis in wild-type cells. Thus, the proteasome is part of the ubiquitin system and mediates the degradation of ubiquitin-protein conjugates in vivo.

引用

页码：3077 / 3080

页数：4

共 46 条

[21] THE MECHANISM AND FUNCTIONS OF ATP-DEPENDENT PROTEASES IN BACTERIAL AND ANIMAL-CELLS [J].

GOLDBERG, AL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (1-2) :9-23

[22] THE PROS-35 GENE ENCODES THE 35KD PROTEIN SUBUNIT OF DROSOPHILA-MELANOGASTER PROTEASOME [J].

HAASS, C ;

PESOLDHURT, B ;

MULTHAUP, G ;

BEYREUTHER, K ;

KLOETZEL, PM .

EMBO JOURNAL, 1989, 8 (08) :2373-2379

[23] PROTEINASE YSCE, THE YEAST PROTEASOME/MULTICATALYTIC-MULTIFUNCTIONAL PROTEINASE - MUTANTS UNRAVEL ITS FUNCTION IN STRESS-INDUCED PROTEOLYSIS AND UNCOVER ITS NECESSITY FOR CELL-SURVIVAL [J].

HEINEMEYER, W ;

KLEINSCHMIDT, JA ;

SAIDOWSKY, J ;

ESCHER, C ;

WOLF, DH .

EMBO JOURNAL, 1991, 10 (03) :555-562

[24] THE SHORT-LIVED MAT-ALPHA-2 TRANSCRIPTIONAL REGULATOR IS UBIQUITINATED INVIVO [J].

HOCHSTRASSER, M ;

ELLISON, MJ ;

CHAU, V ;

VARSHAVSKY, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4606-4610

[25]

HOUGH R, 1986, J BIOL CHEM, V261, P2400

[26]

HOUGH R, 1987, J BIOL CHEM, V262, P8308

[27]

Jentsch S, 1992, Trends Cell Biol, V2, P98, DOI 10.1016/0962-8924(92)90013-D

[28] UBIQUITIN-CONJUGATING ENZYMES - NOVEL REGULATORS OF EUKARYOTIC CELLS [J].

JENTSCH, S ;

SEUFERT, W ;

SOMMER, T ;

REINS, HA .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (05) :195-198

[29] UBIQUITIN AS A DEGRADATION SIGNAL [J].

JOHNSON, ES ;

BARTEL, B ;

SEUFERT, W ;

VARSHAVSKY, A .

EMBO JOURNAL, 1992, 11 (02) :497-505

[30] PROTEINASE YSCE OF YEAST SHOWS HOMOLOGY WITH THE 20S CYLINDER PARTICLES OF XENOPUS-LAEVIS [J].

KLEINSCHMIDT, JA ;

ESCHER, C ;

WOLF, DH .

FEBS LETTERS, 1988, 239 (01) :35-40

← 1 2 3 4 5 →