INVIVO FUNCTION OF THE PROTEASOME IN THE UBIQUITIN PATHWAY

被引:138
作者
SEUFERT, W [1 ]
JENTSCH, S [1 ]
机构
[1] MAX PLANCK GESELL,FRIEDRICH MIESCHER LAB,SPEMANNSTR 37-39,W-7400 TUBINGEN,GERMANY
关键词
PROTEASOME; PROTEIN DEGRADATION; UBIQUITIN SYSTEM; YEAST;
D O I
10.1002/j.1460-2075.1992.tb05379.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A major eukaryotic proteolytic system is known to require the covalent attachment of ubiquitin to substrates prior to their degradation, yet the proteinase involved remains poorly defined. The proteasome, a large conserved multi-subunit protein complex of the cytosol and the nucleus, has been implicated in a variety of cellular functions. It is shown here that a yeast mutant with a defective proteasome fails to degrade proteins which are subject to ubiquitin-dependent proteolysis in wild-type cells. Thus, the proteasome is part of the ubiquitin system and mediates the degradation of ubiquitin-protein conjugates in vivo.
引用
收藏
页码:3077 / 3080
页数:4
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