Anisakis simplex: Stage-specific antigens recognized by mice

被引：11

作者：

Iglesias, R ^{[1
]}

Leiro, J ^{[1
]}

Ubeira, FM ^{[1
]}

Santamarina, MT ^{[1
]}

Sanmartin, ML ^{[1
]}

机构：

[1] UNIV SANTIAGO DE COMPOSTELA,FAC FARM,PARASITOL LAB,INST INVES & ANAL ALIMENTARIO,E-15706 SANTIAGO,SPAIN

来源：

JOURNAL OF HELMINTHOLOGY | 1995年 / 69卷 / 04期

关键词：

D O I：

10.1017/S0022149X00014899

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion-secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS-PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion-secretion and surface antigen preparations from third-stage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion-secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis.

引用

页码：319 / 324

页数：6

共 23 条

[11] HUMAN ANISAKIASIS - AN UPDATE [J].