CLONING, SEQUENCING AND DELETION FROM THE CHROMOSOME OF THE GENE ENCODING SUBUNIT-1 OF THE AA3-TYPE CYTOCHROME-C-OXIDASE OF RHODOBACTER-SPHAEROIDES

被引：68

作者：

SHAPLEIGH, JP ^{[1
]}

GENNIS, RB ^{[1
]}

机构：

[1] UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01511.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% identity with that from Paracoccus denitrificans. The ctaD gene was deleted from the chromosome of R. sphaeroides, resulting in a strain that spectroscopically lacks cytochrome a. This strain maintains about 50% of the cytochrome c oxidase activity of the wild-type strain owing to the presence of an alternate o-type cytochrome c oxidase. The aa3-type oxidase was restored by complementing the chromosomal deletion with a plasmid-borne copy of the ctaD gene. This system is well suited for site-directed mutagenesis probing of the structure and function of cytochrome c oxidase.

引用

页码：635 / 642

页数：8

共 42 条

[41] ASSIGNMENT OF THE HISTIDINE AXIAL LIGANDS TO THE CYTOCHROME-BH AND CYTOCHROME-BL COMPONENTS OF THE BC1 COMPLEX FROM RHODOBACTER-SPHAEROIDES BY SITE-DIRECTED MUTAGENESIS [J].