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SINGLE-PASS SEQUENCING OF A UNIDIRECTIONAL HUMAN FETAL HEART CDNA LIBRARY TO DISCOVER NOVEL GENES OF THE CARDIOVASCULAR-SYSTEM

被引：36
作者：
HWANG, DM
HWANG, WS
LIEW, CC
机构：
[1] UNIV TORONTO, CARDIOVASC RES CTR, MOLEC CARDIOL LAB, TORONTO, ON, CANADA
[2] UNIV TORONTO, DEPT CLIN BIOCHEM, TORONTO, ON, CANADA
[3] UNIV TORONTO, DEPT MED, TORONTO, ON, CANADA
[4] UNIV CALGARY, DEPT PATHOL, CALGARY, AB, CANADA
来源：
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 1994年 / 26卷 / 10期
关键词：
HUMAN HEART CDNA LIBRARY; EXPRESSED SEQUENCE TAGS (ESTS);
D O I：
10.1006/jmcc.1994.1151
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A human fetal heart cDNA library was constructed in the lambda gt22A expression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequencing reactions in the presence of a fluorescein-conjugated primer and electrophoresed on a Pharmacia A.L.F. Sequencer. Partial cDNA sequences, or expressed sequence tags (ESTs) were searched against the Genbank and EMBL databases to identify novel genes expressed in the human cardiovascular system.
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页码：1329 / 1333
页数：5
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