A NOVEL BETA-N-ACETYLGLUCOSAMINIDASE ACTIVITY IN HOG GASTRIC-MUCOSAL MICROSOMES - PREFERENTIAL HYDROLYSIS OF TERMINAL GLCNAC-BETA-1-3 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC, BUT GLCNAC-BETA-1-6 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL

被引：6

作者：

HELIN, J

SEPPO, A

LEPPANEN, A

PENTTILA, L

MAAHEIMO, H

NIEMELA, R

LAURI, S

RENKONEN, O

机构：

[1] UNIV HELSINKI,INST BIOTECHNOL,SF-00014 HELSINKI,FINLAND

[2] UNIV HELSINKI,DEPT BIOCHEM,SF-00014 HELSINKI,FINLAND

来源：

FEBS LETTERS | 1993年 / 335卷 / 02期

关键词：

P-N-ACETYLGLUCOSAMINIDASE; LINKAGE SPECIFICITY; OLIGO-N-ACETYLLACTOSAMINOGLYCAN; IN VITRO SYNTHESIS; HOG GASTRIC MUCOSAL MICROSOME;

D O I：

10.1016/0014-5793(93)80747-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hog gastric mucosal microsomes contain beta-N-acetylglucosaminidase activity which cleaves GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc at the terminal GlcNAc beta 1-3Gal linkage faster than at the GlcNAc beta 1-6Gal bond, producing mainly GlcNAc beta 1-6Gal beta 1-4GlcNAc. In a marked contrast, GlcNAc beta 1-3(GlcNAc beta 1-6)Gal is cleaved primarily at the GlcNAc beta 1-6Gal bond, while partial hydrolysis of GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4Glc reveals similar rates of cleavage for the (1-3) and (1-6) linkages. Our data support the notion that the terminal beta 1,6-linked GlcNAc unit of GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc may interact with the reducing end GlcNAc unit intramolecularly in water solution.

引用

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页码：280 / 284

页数：5

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