PURIFICATION AND PROPERTIES OF ARGINASE FROM SOYBEAN, GLYCINE-MAX, AXES

被引:51
作者
KANG, JH [1 ]
CHO, YD [1 ]
机构
[1] YONSEI UNIV,COLL SCI,DEPT BIOCHEM,SEOUL 120749,SOUTH KOREA
关键词
D O I
10.1104/pp.93.3.1230
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arginase (EC 3.5.3.1) was purified to homogeneity from cytosol of soybean, Glycine max, axes by chromatographic separations on Sephadex G-200, DEAE-sephacel, hydroxyapatite, and arginine-affinity columns. The molecular weight of the enzyme estimated by pore gradient gel electrophoresis was 240,000, while sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecular weight of 60,000. The optimal pH for activity was 9.5 and the Km value was 83 millimolar. The enzyme was stimulated by polyamines such as putrescine.
引用
收藏
页码:1230 / 1234
页数:5
相关论文
共 29 条
[21]   POLYAMINE BIOSYNTHESIS AND TITER DURING VARIOUS DEVELOPMENTAL STAGES OF PHASEOLUS-VULGARIS [J].
PALAVAN, N ;
GALSTON, AW .
PHYSIOLOGIA PLANTARUM, 1982, 55 (04) :438-444
[23]   APPEARANCE OF ARGININE AND ARGINASE IN PUMPKIN COTYLEDONS - CHARACTERIZATION OF ARGINASE [J].
SPLITTST.WE .
PHYTOCHEMISTRY, 1969, 8 (04) :753-&
[24]   POLYAMINES [J].
TABOR, CW ;
TABOR, H .
ANNUAL REVIEW OF BIOCHEMISTRY, 1984, 53 :749-790
[25]  
Talor AA, 1981, BIOCHEM BIOPH RES CO, V101, P1281
[26]   MOLECULAR-FORMS OF RAT-LIVER ARGINASE - ISOLATION AND CHARACTERIZATION [J].
TARRAB, R ;
RODRIGUEZ, J ;
HUITRON, C ;
PALACIOS, R ;
SOBERON, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 49 (02) :457-468
[27]  
VAIDYANATHAN C. S., 1953, ENZYMOLOGIA, V16, P167
[28]   PURIFICATION AND PROPERTIES OF THE ARGINASE FROM JERUSALEM ARTICHOKE TUBERS [J].
WRIGHT, LC ;
BRADY, CJ ;
HINDE, RW .
PHYTOCHEMISTRY, 1981, 20 (12) :2641-2645
[29]   PHYSIOLOGICAL CONTROL OF ARGININE DECARBOXYLASE ACTIVITY IN K-DEFICIENT OAT SHOOTS [J].
YOUNG, ND ;
GALSTON, AW .
PLANT PHYSIOLOGY, 1984, 76 (02) :331-335