EXCESS NONSYNONYMOUS SUBSTITUTION AT SHARED POLYMORPHIC SITES AMONG SELF-INCOMPATIBILITY ALLELES OF SOLANACEAE

被引:116
作者
CLARK, AG
KAO, TH
机构
[1] PENN STATE UNIV,DEPT BIOL,UNIVERSITY PK,PA 16802
[2] PENN STATE UNIV,DEPT MOLEC & CELL BIOL,UNIVERSITY PK,PA 16802
关键词
MOLECULAR EVOLUTION; SHARED POLYMORPHISM;
D O I
10.1073/pnas.88.21.9823
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The function of the self-incompatibility locus (S locus) of many plant species dictates that natural selection will favor high levels of protein diversity. Pairwise sequence comparisons between S alleles from four species of Solanaceae reveal remarkably high sequence diversity and evidence for shared polymorphism. The level of amino acid constraint was found to be significantly heterogeneous among different regions of the gene, with some regions being highly constrained and others appearing to be virtually unconstrained. In some regions of the protein, there was an excess of nonsynonymous over synonymous substitution, consistent with the strong diversifying selection that must operate on this locus. These hypervariable regions are candidates for the sites that determine functional allelic identity. Simple contingency table tests show that sites that have polymorphism shared between species have more nonsynonymous substitution than polymorphic sites that do not exhibit shared polymorphism. This is consistent with the idea that adaptive evolution favoring amino acid replacement is occurring at sites with shared polymorphism. Tests of clustered polymorphism reveal that an unusually low rate of recombination must be occurring in this locus, allowing very ancient alleles to preserve their identity.
引用
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页码:9823 / 9827
页数:5
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