MIXED CARBON PASTE PEA SEEDLING ELECTROCHEMICAL SENSOR FOR MEASURING PLANT GROWTH-REGULATING ACTIVITY OF AMINES

The use of pea seedlings as a source of diamine oxidase for the determination of enzymatic oxidation rates, which are important in evaluating the plant growth-regulating activity, of various amines is reported. A mixed carbon paste-plant tissue amperometric sensor was constructed and characterized using speramidine as a substrate (K(M) = 5 x 10(-6) M). Generation of hydrogen peroxide due to enzymatic oxidation of amine was monitored at 0.9 V vs. Ag/AgCl under steady-state conditions using this sensor. Easy construction, reliability of the data, reusability, shorter response times (of the order of 4 s) and a detection limit of 7.1 nmol 1(-1) are some of the advantages.