PHOTOCHEMICAL CROSS-LINKING OF THE ANTICODON LOOP OF YEAST TRANSFER RNA(PHE) TO 30S-SUBUNIT PROTEIN-S7 AT THE RIBOSOMAL-A AND RIBOSOMAL-P SITES

被引：29

作者：

SYLVERS, LA

KOPYLOV, AM

WOWER, J

HIXSON, SS

ZIMMERMANN, RA

机构：

[1] UNIV MASSACHUSETTS, PROGRAM MOLEC & CELLULAR BIOL, AMHERST, MA 01003 USA

[2] UNIV MASSACHUSETTS, DEPT BIOCHEM & MOLEC BIOL, AMHERST, MA 01003 USA

[3] UNIV MASSACHUSETTS, DEPT CHEM, AMHERST, MA 01003 USA

[4] MV LOMONOSOV STATE UNIV, BELOZERSKY LAB PHYSICOCHEM BIOL, MOSCOW 119899, USSR

来源：

BIOCHIMIE | 1992年 / 74卷 / 04期

关键词：

TRANSFER RNA-RIBOSOME INTERACTION; DECODING SITE; TRANSFER RNA-PROTEIN CROSS-LINK; 30S SUBUNIT MODEL; AZIDOADENOSINE; PHOTOAFFINITY LABELING;

D O I：

10.1016/0300-9084(92)90116-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast tRNA(Phe), containing the photoreactive nucleoside 2-azidoadenosine at position 37 within the anticodon loop, has been cross-linked to the aminoacyl-tRNA (A) and peptidyl-tRNA (P) binding sites of the Escherichia coli ribosome. The 30S subunit was exclusively labeled in each case, and cross-linking occurred to both protein and 16S rRNA. Electrophoretic and immunological analyses demonstrated that S7 was the only 30S-subunit protein covalently attached to the tRNA. However, digestion of the A and P site-labeled S7 with trypsin revealed a unique pattern of cross-linked peptide(s) at each site. Thus, while the anticodon loop of tRNA is in close proximity to protein S7 at both the A and P sites, it neighbors a different portion of the protein molecule in each. The placement of the aminoacyl- and peptidyl-tRNA binding sites is discussed in relationship to recent models of the 30S ribosomal subunit.

引用

页码：381 / 389

页数：9

共 42 条

[1] NUCLEOTIDE RESIDUES OF TRANSFER-RNA, DIRECTLY INTERACTING WITH PROTEINS WITHIN THE COMPLEX OF THE 30-S SUBUNIT OF ESCHERICHIA-COLI RIBOSOME WITH POLY(U) AND NACPHE-TRANSFER RNAPHE [J].