PURIFICATION AND CHARACTERIZATION OF OXYGEN-EVOLVING PHOTOSYSTEM-II CORE COMPLEXES FROM THE GREEN-ALGA CHLAMYDOMONAS-REINHARDTII

被引：18

作者：

BUMANN, D ^{[1
]}

OESTERHELT, D ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,DEPT MEMBRANE BIOCHEM,D-82152 MARTINSRIED,GERMANY

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 36期

关键词：

D O I：

10.1021/bi00202a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxygen-evolving photosystem II complexes were isolated from the green alga Chlamydomonas reinhardtii by selective solubilization of thylakoid membranes with dodecyl maltoside followed by density gradient centrifugation and anion-exchange chromatography. In the presence of CaCl2 and K-3[Fe(CN)(6)] the complexes evolved oxygen at rates exceeding 1000 mu mol (mg of chl)(-1) h(-1). The particles contained 40 chlorophylls a and had properties very similar to those of PSII isolated from higher plants. Chlamydomonas reinhardtii is now the first organism which can be used for both site-directed mutagenesis and detailed biochemical and biophysical characterization of oxygen-evolving photosystem II. It seems therefore to be an ideal model organism for investigation of structure-function relationships in photosynthetic oxygen evolution.

引用

页码：10906 / 10910

页数：5