AMPEROMETRIC ENZYME ELECTRODE FOR THE DETERMINATION OF ASPARTAME IN DIET FOOD

被引：10

作者：

VILLARTA, RL ^{[1
]}

SULEIMAN, AA ^{[1
]}

GUILBAULT, GG ^{[1
]}

机构：

[1] UNIV NEW ORLEANS,DEPT CHEM,NEW ORLEANS,LA 70148

来源：

MICROCHEMICAL JOURNAL | 1993年 / 48卷 / 01期

关键词：

D O I：

10.1006/mchj.1993.1071

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric enzyme electrode for the determination of aspartame in protein-free samples has been constructed. It is based on the coimmobilization of an aspartame-hydrolyzing enzyme, aspartate aminotransferase, and glutamate oxidase on an Immobilon membrane. The electrode response is linear from 2.0 × 10-4M to 1.5 × 10-3M with a current slope of 2.1 nA/mM. The electrode was used to successfully measure aspartame in some dietary products. © 1993 Academic Press. All rights reserved.

引用

收藏

页码：60 / 64

页数：5

相关论文

共 17 条

[1] ISOCRATIC LIQUID-CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS DETERMINATION OF ASPARTAME AND OTHER ADDITIVES IN SOFT DRINKS [J].

ARGOUDELIS, CJ .

JOURNAL OF CHROMATOGRAPHY, 1984, 303 (01) :256-262

[2] ASPARTYLPHENYLALANINE METHYL ESTER - A LOW-CALORIE SWEETENER [J].

CLONINGER, MR ;

BALDWIN, RE .

SCIENCE, 1970, 170 (3953) :81-+

[3] BIENZYMATIC ELECTRODE FOR THE DETERMINATION OF ASPARTAME IN DIETARY PRODUCTS [J].

FATIBELLOFILHO, O ;

SULEIMAN, AA ;

GUILBAULT, GG ;

LUBRANO, GJ .

ANALYTICAL CHEMISTRY, 1988, 60 (21) :2397-2399

[4]

GUILBAULT GG, 1988, ANAL CHIM ACTA, V206, P369, DOI 10.1016/S0003-2670(00)80859-4

[5] THE DETERMINATION OF ASPARTAME IN DIET SOFT DRINKS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

ISSAQ, HJ ;

WEISS, D ;

RIDLON, C ;

FOX, SD ;

MUSCHIK, GM .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1986, 9 (08) :1791-1802

[6]

JOHNS DR, 1986, NEW ENGL J MED, V315, P456

[7] DETERMINATION OF ASPARTAME IN DIETARY FOOD-PRODUCTS BY A FIA BIOSENSOR [J].

MALE, KB ;

LUONG, JHT ;

MULCHANDANI, A .

BIOSENSORS & BIOELECTRONICS, 1991, 6 (02) :117-123

[8] STRUCTURE-TASTE RELATIONSHIPS OF SOME DIPEPTIDES [J].

MAZUR, RH ;

SCHLATTER, JM ;

GOLDKAMP, AH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (10) :2684-+

[9] ENZYMATIC ASSAY TECHNIQUE FOR THE DETERMINATION OF ASPARTAME [J].

MULCHANDANI, A ;

MALE, KB ;

LUONG, JHT ;

GIBBS, BF .

ANALYTICA CHIMICA ACTA, 1990, 234 (02) :465-469

[10] ALTERATION OF SUBSTRATE REGULATION PATTERNS IN GLUTAMATE-DEHYDROGENASE BY ENZYME IMMOBILIZATION [J].

OCONNOR, KC ;

SCHUTZ, HJ ;

BAILEY, JE .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (07) :896-905