SEQUENCE-ANALYSIS OF THE PROMOTER REGION OF THE RAT SOMATOSTATIN RECEPTOR SUBTYPE-1 GENE

被引：46

作者：

HAUSER, F ^{[1
]}

MEYERHOF, W ^{[1
]}

WULFSEN, I ^{[1
]}

SCHONROCK, C ^{[1
]}

RICHTER, D ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ZELLBIOCHEM & KLIN NEUROBIOL,UKE,D-20246 HAMBURG,GERMANY

来源：

FEBS LETTERS | 1994年 / 345卷 / 2-3期

关键词：

PIT-1; PITUITARY; GCF; AP-2;

D O I：

10.1016/0014-5793(94)00444-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Somatostatin receptor (SSTR) subtype genes are differentially expressed in brain and various peripheral tissues. RNA blotting and semiquantitative PCR analyses have revealed low levels of SSTR1 mRNA in the gastrointestinal tract and relatively high levels in GH(3) anterior pituitary cells. As a first step in the investigation of the regulation of SSTR1 gene expression, we isolated a genomic fragment that contains the promoter region and determined the transcriptional initiation site. The SSTR1 gene lacks introns and TATA and CAAT motifs, but possesses several consensus recognition sequences for the transcription factors GCF and AP-2. The presence, also, of two Pit-1 binding sites could explain the high SSTR1 mRNA levels in GH(3) cells.

引用

页码：225 / 228

页数：4

共 26 条

[11] CHARACTERIZATION AND SEQUENCE OF THE PROMOTER REGION OF THE HUMAN EPIDERMAL GROWTH-FACTOR RECEPTOR GENE [J].

ISHII, S ;

XU, YH ;

STRATTON, RH ;

ROE, BA ;

MERLINO, GT ;

PASTAN, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :4920-4924

[12] PROMOTER REGION OF THE HUMAN HARVEY RAS PROTO-ONCOGENE - SIMILARITY TO THE EGF RECEPTOR PROTO-ONCOGENE PROMOTER [J].

ISHII, S ;

MERLINO, GT ;

PASTAN, I .

SCIENCE, 1985, 230 (4732) :1378-1381

[13] MOLECULAR-CLONING AND CHARACTERIZATION OF A HUMAN DNA-BINDING FACTOR THAT REPRESSES TRANSCRIPTION [J].

KAGEYAMA, R ;

PASTAN, I .

CELL, 1989, 59 (05) :815-825

[14] A PITUITARY POU DOMAIN PROTEIN, PIT-1, ACTIVATES BOTH GROWTH-HORMONE AND PROLACTIN PROMOTERS TRANSCRIPTIONALLY [J].

MANGALAM, HJ ;

ALBERT, VR ;

INGRAHAM, HA ;

KAPILOFF, M ;

WILSON, L ;

NELSON, C ;

ELSHOLTZ, H ;

ROSENFELD, MG .

GENES & DEVELOPMENT, 1989, 3 (07) :946-958

[15]

Maniatis T., 1982, MOL CLONING

[16] CLONING OF A CDNA-ENCODING A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR EXPRESSED IN SPECIFIC RAT-BRAIN REGIONS [J].

MEYERHOF, W ;

PAUST, HJ ;

SCHONROCK, C ;

RICHTER, D .

DNA AND CELL BIOLOGY, 1991, 10 (09) :689-694

[17] MOLECULAR-CLONING OF A SOMATOSTATIN-28 RECEPTOR AND COMPARISON OF ITS EXPRESSION PATTERN WITH THAT OF A SOMATOSTATIN-14 RECEPTOR IN RAT-BRAIN [J].

MEYERHOF, W ;

WULFSEN, I ;

SCHONROCK, C ;

FEHR, S ;

RICHTER, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10267-10271

[18]

OCARROLL AM, 1992, MOL PHARMACOL, V42, P939

[19] FINE-STRUCTURE OF THE HUMAN HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE GENE [J].

PATEL, PI ;

FRAMSON, PE ;

CASKEY, CT ;

CHINAULT, AC .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) :393-403

[20]

RAYNOR K, 1991, MOL PHARMACOL, V40, P248

← 1 2 3 →