THERMOSPRAY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MASS-SPECTRAL ANALYSES OF THE PHOTOPRODUCTS OF DTPDT AND DTPDU

被引：6

作者：

BERUBE, R

LEMAIRE, DGE

RUZSICSKA, BP

机构：

[1] UNIV SHERBROOKE, FAC MED, DEPT MED NUCL & RADIOBIOL, SHERBROOKE J1H 5N4, QUEBEC, CANADA

[2] CHU SHERBROOKE, CTR RECH CLIN, SPECTROMETRIE MASSE LAB, SHERBROOKE J1H 5N4, QUEBEC, CANADA

来源：

BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 05期

关键词：

D O I：

10.1002/bms.1200210507

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The photoproducts of the dinucleoside monophosphates dTpdT and dTpdU have been examined by thermospray high-performance liquid chromatography/mass spectrometry in the positive and negative ion modes. The photoproducts included the cis-syn and trans-syn cyclobutane dimers, the 6-4 and Dewar 6-4 pyrimidine-pyrimidinone photoadducts and the uracil photohydrates of dTpdU. Molecular ions and several fragment ions are observed in all cases. It is shown that the cyclobutane dimers, 6-4 photoadducts and Dewar 6-4 photoadducts can be easily distinguished from each other and from the parent dinucleoside monophosphates by fragmentation pattern differences. Using single-ion monitoring these photoproducts could be detected st the low-picomole level.

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页码：259 / 266

页数：8

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