SELF-STABILIZED ANTISENSE OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES - PROPERTIES AND ANTI-HIV ACTIVITY

被引：100

作者：

TANG, JY ^{[1
]}

TEMSAMANI, J ^{[1
]}

AGRAWAL, S ^{[1
]}

机构：

[1] HYBRIDON INC, 1 INNOVAT DR, WORCESTER, MA 01605 USA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 11期

关键词：

D O I：

10.1093/nar/21.11.2729

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new class of oligodeoxyribonucleotides has been designed, referred to here as 'self-stabilized' oligonucleotides. These oligonucleotides have hairpin loop structures at their 3' ends, and show increased resistance to degradation by snake venom phosphodiesterase, DNA polymerase I and fetal bovine serum. The self-stabilized region of the oligonucleotide does not interfere In hybridization with complementary nucleic acids as shown by melting temperature, mobility-shift and RNase H cleavage studies. Various self-stabilized oligonucleotides containing increasingly stable hairpin loop regions were studied for their anti-HIV activity. Pharmacokinetic and stability studies in mice showed increased in vivo persistence of self-stabilized oligonucleotides with respect to their linear counterparts.

引用

页码：2729 / 2735

页数：7

共 29 条

[1] PHARMACOKINETICS, BIODISTRIBUTION, AND STABILITY OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES IN MICE [J].