MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HUMAN DIPEPTIDYL PEPTIDASE-IV, A SERINE PROTEINASE ON THE CELL-SURFACE

被引：102

作者：

MISUMI, Y ^{[1
]}

HAYASHI, Y ^{[1
]}

ARAKAWA, F ^{[1
]}

IKEHARA, Y ^{[1
]}

机构：

[1] FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,JONAN KU,FUKUOKA 81401,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 03期

关键词：

DIPEPTIDYL PEPTIDASE-IV; CLONING; SEQUENCE ANALYSIS; (HUMAN);

D O I：

10.1016/0167-4781(92)90036-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA coding for the human dipeptidyl peptidase IV (DPPIV) has been isolated and sequenced. The nucleotide sequence (3465 bp) of the cDNA contains an open reading frame encoding a polypeptide comprising 766 amino acids, one residue less than those of rat DPPIV. The predicted amino acid sequence exhibits 84.9% identity to that of the rat enzyme, and contains nine potential N-linked glycosylation sites, one site more than those in the rat enzyme. A putative catalytic triad for serine proteinases, serine, aspartic acid and histidine, are found in a completely conserved COOH-terminal region (positions 625-752).

引用

页码：333 / 336

页数：4

共 27 条

[1] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].

BRADY, L ;

BRZOZOWSKI, AM ;

DEREWENDA, ZS ;

DODSON, E ;

DODSON, G ;

TOLLEY, S ;

TURKENBURG, JP ;

CHRISTIANSEN, L ;

HUGEJENSEN, B ;

NORSKOV, L ;

THIM, L ;

MENGE, U .

NATURE, 1990, 343 (6260) :767-770

[2] PRIMARY STRUCTURE AND ENZYMATIC-PROPERTIES OF CARBOXYPEPTIDASE-II FROM WHEAT BRAN [J].

BREDDAM, K ;

SORENSEN, SB ;

SVENDSEN, I .

CARLSBERG RESEARCH COMMUNICATIONS, 1987, 52 (04) :297-311

[3] THE MOLECULAR EVOLUTION OF GENES AND PROTEINS - A TALE OF 2 SERINES [J].

BRENNER, S .

NATURE, 1988, 334 (6182) :528-530

[4] SIMPLIFIED PREPARATION OF UNIDIRECTIONAL DELETION CLONES [J].

HOHEISEL, J ;

POHL, FM .

NUCLEIC ACIDS RESEARCH, 1986, 14 (08) :3605-3605

[5] PROTEASE-II FROM ESCHERICHIA-COLI - SEQUENCING AND EXPRESSION OF THE ENZYME GENE AND CHARACTERIZATION OF THE EXPRESSED ENZYME [J].

KANATANI, A ;

MASUDA, T ;

SHIMODA, T ;

MISOKA, F ;

LIN, XS ;

YOSHIMOTO, T ;

TSURU, D .

JOURNAL OF BIOCHEMISTRY, 1991, 110 (03) :315-320

[6] DIPEPTIDYL PEPTIDASEIV, A KIDNEY BRUSH-BORDER SERINE PEPTIDASE [J].

KENNY, AJ ;

BOOTH, AG ;

GEORGE, SG ;

INGRAM, J ;

KERSHAW, D ;

WOOD, EJ ;

YOUNG, AR .

BIOCHEMICAL JOURNAL, 1976, 157 (01) :169-+

[7]

Kraut J, 1971, ENZYMES, V3, P547

[8] PROTEINS OF THE KIDNEY MICROVILLAR MEMBRANE - AMPHIPATHIC FORM OF DIPEPTIDYL PEPTIDASE-IV [J].

MACNAIR, RDC ;

KENNY, AJ .

BIOCHEMICAL JOURNAL, 1979, 179 (02) :379-+

[9]

MANIATIS T, 1982, MOL CLONING LABORATO, P368

[10]

MARQUET D, 1992, J BIOL CHEM, V267, P2200

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