CARBOHYDRATE STRUCTURES OF THE GLYCOPROTEIN ALLERGEN CRY-J-I FROM JAPANESE CEDAR (CRYPTOMERIA-JAPONICA) POLLEN

The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides, The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine, The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC, Their structures were determined by sequential exoglycosidase digestion and 500 MHz H-1-NMR spectroscopy, Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type, They were present at a molar ratio of 8:1:6:1, By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides. [GRAPHICS]