QUANTITATIVE-ANALYSIS OF RIBONUCLEOTIDE TRIPHOSPHATES IN CELL-EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY - A COMPARATIVE-STUDY

被引：18

作者：

LOREGIAN, A

SCREMIN, C

SCHIAVON, M

MARCELLO, A

PALU, G

机构：

[1] UNIV PADUA,INST MICROBIOL,I-35121 PADUA,ITALY

[2] BECKMAN ANALYT SPA,I-20060 CASSINA DE PECCHI,ITALY

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 18期

关键词：

D O I：

10.1021/ac00090a028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two analytical methods have been evaluated for their ability to separate and quantitate the intracellular pool of free ribonucleotide triphosphates in four different cell lines. Micellar electrokinetic capillary chromatography and highperformance liquid chromatography were compared in terms of speed, sensitivity, and efficiency of analysis, with the former being 3 times faster (about 10 vs 30 min) and having an average minimun detectable quantity of 50 fmol vs 30 pmol and approximately 950 000 theorical plates/m vs 4500. Different protocols of extraction of nucleotides from whole cells were also evaluated with the two analytical methods. Micellar electrokinetic capillary chromatography proved to be a powerful tool for the reproducible and reliable the fast processing of extremely small volumes of sample.

引用

页码：2981 / 2984

页数：4

共 27 条

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