A MODEL ONLINE FLOW-INJECTION FLUORESCENCE IMMUNOASSAY USING A PROTEIN-A IMMUNOREACTOR AND LUCIFER YELLOW

被引：22

作者：

PALMER, DA

XUEZHEN, R

FERNANDEZHERNANDO, P

MILLER, JN

机构：

[1] Department of Chemistry, Loughborough University of Technology, Loughborough

[2] Department of Chemistry, Shandong University

[3] Universidad Complutense de Madrid

来源：

ANALYTICAL LETTERS | 1993年 / 26卷 / 12期

关键词：

TRANSFERRIN; HETEROGENEOUS FLUORESCENCE IMMUNOASSAY; FLOW INJECTION ANALYSIS; PROTEIN-A; LUCIFER YELLOW;

D O I：

10.1080/00032719308017972

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A heterogeneous on-line fluorescence immunoassay has been developed for a model analyte (transferrin) using a flow injection analysis system containing a controlled pore glass protein A immunoreactor. Lucifer yellow VS (LYVS) a 4-aminonaphthalimide with a large stokes shift and pH independence was the fluorophore. For each assay the antibody-protein A reaction takes place at near neutral pH, and the complexes are eluted at acid pH. Transferrin levels in human serum has been determined by this method, and good within assay variations have been achieved.

引用

页码：2543 / 2553

页数：11

共 17 条

[1] DETERMINATION OF SPECIFIC PROTEINS BY THE FIA PRINCIPLE [J].