AN EXTENDED PANEL OF HAMSTER-HUMAN HYBRIDS FOR CHROMOSOME 2Q

被引：4

作者：

HAFEZPARAST, M

COLE, CG

KAUR, GP

ATHWAL, RS

JEGGO, PA

机构：

[1] SANGER CTR,CAMBRIDGE CB10 1RQ,ENGLAND

[2] TEMPLE UNIV,SCH MED,FELS INST CANC RES & MOLEC BIOL,PHILADELPHIA,PA 19140

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1994年 / 20卷 / 06期

关键词：

D O I：

10.1007/BF02255844

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A hamster-human hybrid containing only the q arm of chromosome 2 has been used to construct a panel of hybrids bearing reduced regions of chromosome 2 using the technique of irradiation fusion gene transfer. The human chromosome 2 carried the Ecogpt gene and all hybrids were selected using this marker. The integrated Ecogpt gene was localized to the region 2q33-34, resulting in the selective retention of this region in the hybrids. These data were combined with another previously constructed panel of hybrids containing regions of 2q, which were enriched for the region 2q36-37. The combined hybrid panel is useful for the mapping of new markers to defined regions of chromosome 2 and for the cloning of genes located on 2q by a positional strategy.

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页码：541 / 548

页数：8

相关论文

共 27 条

[1] A YEAST ARTIFICIAL CHROMOSOME CONTIG ENCOMPASSING THE CYSTIC-FIBROSIS LOCUS [J].

ANAND, R ;

OGILVIE, DJ ;

BUTLER, R ;

RILEY, JH ;

FINNIEAR, RS ;

POWELL, SJ ;

SMITH, JC ;

MARKHAM, AF .

GENOMICS, 1991, 9 (01) :124-130

[2] INTEGRATION OF A DOMINANT SELECTABLE MARKER INTO HUMAN-CHROMOSOMES AND TRANSFER OF MARKED CHROMOSOMES TO MOUSE CELLS BY MICROCELL FUSION [J].

ATHWAL, RS ;

SMARSH, M ;

SEARLE, BM ;

DEO, SS .

SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (02) :177-187

[3]

ATHWAL RS, 1984, GENE TRANSFER CANCER, P21

[4] A HIGHLY INFORMATIVE DINUCLEOTIDE REPEAT POLYMORPHISM AT THE D2S211 LOCUS LINKED TO ALPP, FN1 AND TNP1 [J].

BARBER, TD ;

MORELL, R ;

JOHNSON, DH ;

ASHER, JH ;

FRIEDMAN, TB .

HUMAN MOLECULAR GENETICS, 1993, 2 (01) :88-88

[5] REARRANGEMENT OF THE PAX3 PAIRED BOX GENE IN THE PEDIATRIC SOLID TUMOR ALVEOLAR RHABDOMYOSARCOMA [J].

BARR, FG ;

GALILI, N ;

HOLICK, J ;

BIEGEL, JA ;

ROVERA, G ;

EMANUEL, BS .

NATURE GENETICS, 1993, 3 (02) :113-117

[6] CONSTRUCTION OF A 2.6-MB CONTIG IN YEAST ARTIFICIAL CHROMOSOMES SPANNING THE HUMAN DYSTROPHIN GENE USING AN STS-BASED APPROACH [J].

COFFEY, AJ ;

ROBERTS, RG ;

GREEN, ED ;

COLE, CG ;

BUTLER, R ;

ANAND, R ;

GIANNELLI, F ;

BENTLEY, DR .

GENOMICS, 1992, 12 (03) :474-484

[7] A RANDOM STS STRATEGY FOR CONSTRUCTION OF YAC CONTIGS SPANNING DEFINED CHROMOSOMAL REGIONS [J].

COLE, CG ;

DUNHAM, I ;

COFFEY, AJ ;

ROSS, MT ;

MEIEREWERT, S ;

BOBROW, M ;

BENTLEY, DR .

GENOMICS, 1992, 14 (02) :256-262

[8] RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES [J].

COX, DR ;

BURMEISTER, M ;

PRICE, ER ;

KIM, S ;

MYERS, RM .

SCIENCE, 1990, 250 (4978) :245-250

[9] A SIMPLE METHOD FOR ORDERING LOCI USING DATA FROM RADIATION HYBRIDS [J].

FALK, CT .

GENOMICS, 1991, 9 (01) :120-123

[10] THE USE OF IRRADIATION AND FUSION GENE-TRANSFER (IFGT) HYBRIDS TO ISOLATE DNA CLONES FROM HUMAN-CHROMOSOME REGION 9Q33-Q34 [J].

FLORIAN, F ;

HORNIGOLD, N ;

GRIFFIN, DK ;

DELHANTY, JD ;

SEFTON, L ;

ABBOTT, C ;

JONES, C ;

GOODFELLOW, PN ;

WOLFE, J .

SOMATIC CELL AND MOLECULAR GENETICS, 1991, 17 (05) :445-453