DIFFERENTIAL SUBCELLULAR-LOCALIZATION OF 2 POPULATIONS OF GLUTAMATE/AMPA RECEPTORS IN THE RAT TELENCEPHALON

被引:22
作者
STANDLEY, S [1 ]
IRVIN, N [1 ]
BAUDRY, M [1 ]
机构
[1] UNIV SO CALIF,NEUROSCI PROGRAM,LOS ANGELES,CA 90089
关键词
D O I
10.1016/0197-0186(94)90072-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The distribution of glutamate AMPA receptors in the synaptosomal and microsomal fractions of neonatal and adult rat telencephalon was studied by determining the saturation kinetics at equilibrium of H-3-AMPA and H-3-CNQX binding. Al both ages, synaptosomal preparations exhibited two populations of H-3-AMPA binding sites with a small number of high affinity sites and a large number of low affinity sites. H-3-AMPA binding to microsomal preparations from both neonatal acid adult rat telencephalon exhibited a much higher proportion of high affinity relative to low affinity sites. H-3-CNQX binding to the same fractions did not parallel H-3-AMPA binding, but was correlated with the low affinity H-3-AMPA binding and with a marker of plasma membranes. The results suggest that nonsynaptic glutamate/AMPA receptors have a high affinity for agonist and become low affinity when inserted into postsynaptic membranes and that H-3-CNQX binds synaptic but not nonsynaptic glutamate/AMPA receptors with high affinity.
引用
收藏
页码:287 / 293
页数:7
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