IDENTIFICATION AND ANALYSIS OF THE GAS VESICLE GENE-CLUSTER ON AN UNSTABLE PLASMID OF HALOBACTERIUM-HALOBIUM

被引:17
作者
DASSARMA, S
机构
[1] Department of Microbiology, University of Massachusetts, Amherst, 01003, Massachusetts
来源
EXPERIENTIA | 1993年 / 49卷 / 6-7期
关键词
HALOBACTERIA; PLASMID; INSERTION SEQUENCE; GAS VESICLE; BIOTECHNOLOGY;
D O I
10.1007/BF01955149
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In our efforts to elucidate the mechanism of high-frequency mutation of Halobacterium halobium to a gas vesicle deficient state, we discovered insertions, deletions, inversions, and complex DNA rearrangements associated with a large endogenous plasmid, pNRC100. The rearrangements are mostly IS element-mediated, and when they occur in a region of pNCRC100 containing a cluster of thirteen genes, gas vesicle mutants result. We have characterized the structure and expression of this gas vesicle protein (gvp) gene cluster and demonstrated its requirement for gas vesicle synthesis and cell flotation by genetic transformation.
引用
收藏
页码:482 / 486
页数:5
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