NO EFFECT OF TREATMENT WITH CARCINOGENS ON CYTOSINE METHYLATION OF MITOCHONDRIAL-DNA ISOLATED FROM RAT ORGANS BY PHENOL-FREE ALKALINE EXTRACTION

被引：8

作者：

BALANSKY, R

NOVIKOV, L

GIANNONI, P

IZZOTTI, A

DEFLORA, S

机构：

[1] UNIV GENOA,INST HYG & PREVENT MED,I-16132 GENOA,ITALY

[2] NATL ONCOL CTR,BU-1756 SOFIA,BULGARIA

[3] NN PETROVS INST ONCOL,ST PETERSBURG 188646,RUSSIA

[4] UNIV GENOA,INST GEN PHYSIOL,I-16132 GENOA,ITALY

[5] UNIV GENOA,INST HYG & PREVENT MED,I-16132 GENOA,ITALY

来源：

CANCER LETTERS | 1995年 / 97卷 / 01期

关键词：

MITOCHONDRIAL DNA; ALKALINE EXTRACTION; RESTRICTION ANALYSIS; CIGARETTE SMOKE; BENZO[A]PYRENE; 2-ACETYLAMINOFLUORENE; DIETHYLNITROSAMINE;

D O I：

10.1016/0304-3835(95)03943-Q

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Due to the postulated role of mitochondrial DNA damage in aging as well as in the pathogenesis of several chronic degenerative diseases, including tumors, there is a need for easy, safe, rapid and inexpensive methods for mitochondrial DNA isolation, We propose a simple, 1-day protocol based on alkaline extraction, which avoids time-consuming gradient centrifugations and use of toxic phenol. This procedure was used for the recovery of mitochondrial DNA from different rat organs (liver, kidney, heart, lung, brain and testis). The yield was quite high. Purity was sufficient enough to perform restriction analyses with several endonucleases. No changes were observed in the structure or methylation patterns of -CCGG- sites in liver mitochondrial DNA isolated from rats exposed whole-body to mainstream cigarette smoke or treated with the carcinogens benzo[a]pyrene, 2-acetylaminofluorene or diethylnitrosamine.

引用

页码：17 / 23

页数：7

共 36 条

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