CAPILLARY ELECTROPHORESIS METHODS FOR A CLEAR IDENTIFICATION OF SELENO AMINO-ACIDS IN COMPLEX MATRICES LIKE HUMAN-MILK

Seleno amino acids have been identified in SEC fractions of human milk and quantified by capillary electrophoresis. For this purpose different CE-methods have been developed to separate these seleno amino acids (Se-R-NH3) from other molecules with similar molecular weights. Besides, methods have been introduced for the exact identification of the analytes: These methods allow the identification of Se-R-NH3 in presence of molecules of nearly equal mobility and overcome identification problems caused by the shifts of migration times (due to different ionic composition) compared to standard solutions. Procedures for different levels of ''identification securities'' have been ruled out and their ''efficiency'' has been discussed.