RAPID RAPD ANALYSIS FOR DISTINGUISHING LISTERIA SPECIES AND LISTERIA-MONOCYTOGENES SEROTYPES USING A CAPILLARY AIR THERMAL CYCLER

被引：13

作者：

BLACK, SF

GRAY, DI

FENLON, DR

KROLL, RG

机构：

[1] CSL,FOOD SCI LAB,BOYD ORR RES CTR,ABERDEEN AB9 8DG,SCOTLAND

[2] SCOTTISH AGR COLL,ABERDEEN,SCOTLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1995年 / 20卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1995.tb00424.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serotypes were produced from serotypes 1a, 2, 3a, 4ab, 4a and 4c but serotypes, 1/2a, 4b, 4d and 7 had similar profiles.

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页码：188 / 190

页数：3

相关论文

共 11 条

[1] DIFFERENTIATION OF LISTERIA-MONOCYTOGENES, LISTERIA-INNOCUA, LISTERIA-IVANOVII, AND LISTERIA-SEELIGERI BY PULSED-FIELD GEL-ELECTROPHORESIS [J].

HOWARD, PJ ;

HARSONO, KD ;

LUCHANSKY, JB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) :709-712

[2] USE OF RIBOSOMAL-RNA GENE RESTRICTION PATTERNS FOR THE IDENTIFICATION OF LISTERIA SPECIES [J].

JACQUET, C ;

AUBERT, S ;

ELSOLH, N ;

ROCOURT, J .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (01) :42-46

[3] DEVELOPMENT OF A RANDOM AMPLIFICATION OF POLYMORPHIC DNA TYPING METHOD FOR LISTERIA-MONOCYTOGENES [J].

LAWRENCE, LM ;

HARVEY, J ;

GILMOUR, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (09) :3117-3119

[4] TYPING OF LISTERIA SPP BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS [J].

MACGOWAN, AP ;

ODONAGHUE, K ;

NICHOLLS, S ;

MCLAUGHLIN, J ;

BENNETT, PM ;

REEVES, DS .

JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (05) :322-327

[5] RAPID ANALYSIS OF CAMPYLOBACTER ISOLATES - DNA FINGERPRINTING WITHOUT THE NEED TO PURIFY DNA [J].

MAZURIER, S ;

VANDEGIESSEN, A ;

HEUVELMAN, K ;

WERNARS, K .

LETTERS IN APPLIED MICROBIOLOGY, 1992, 14 (06) :260-262

[6] A COMPARATIVE-STUDY OF RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND CONVENTIONAL PHAGE TYPING FOR EPIDEMIOLOGIC STUDIES OF LISTERIA-MONOCYTOGENES ISOLATES [J].

MAZURIER, SI ;

AUDURIER, A ;

MARQUETVANDERMEE, N ;

NOTERMANS, S ;

WERNARS, K .

RESEARCH IN MICROBIOLOGY, 1992, 143 (05) :507-512

[7] TYPING OF LISTERIA STRAINS BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA [J].

MAZURIER, SI ;

WERNARS, K .

RESEARCH IN MICROBIOLOGY, 1992, 143 (05) :499-505

[8] CHARACTERIZATION OF BACILLUS-LICHENIFORMIS WITH THE RAPD TECHNIQUE (RANDOMLY AMPLIFIED POLYMORPHIC DNA) [J].

STEPHAN, R ;

SCHRAFT, H ;

UNTERMANN, F .

LETTERS IN APPLIED MICROBIOLOGY, 1994, 18 (05) :260-263

[9]

WITTWER C, 1994, RAPID CYCLIST, V2, P11

[10]

WITTWER CT, 1991, BIOTECHNIQUES, V10, P76