IMMUNOHISTOCHEMICAL LOCALIZATION OF A METABOTROPIC GLUTAMATE-RECEPTOR, MGLUR5, IN THE RAT-BRAIN

被引：482

作者：

SHIGEMOTO, R

NOMURA, S

OHISHI, H

SUGIHARA, H

NAKANISHI, S

MIZUNO, N

机构：

[1] KYOTO UNIV,FAC MED,INST IMMUNOL,SAKYO KU,KYOTO 606,JAPAN

[2] KYOTO UNIV,COLL MED TECHNOL,SAKYO KU,KYOTO 606,JAPAN

来源：

NEUROSCIENCE LETTERS | 1993年 / 163卷 / 01期

关键词：

METABOTROPIC GLUTAMATE RECEPTOR; MGLUR5; ANTIBODY; IMMUNOHISTOCHEMISTRY; ELECTRON MICROSCOPY; RAT; BRAIN;

D O I：

10.1016/0304-3940(93)90227-C

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A trpE-fusion protein containing a C-terminal sequence of a rat metabotropic glutamate receptor, mGluR5, was used to produce an antibody. On immunoblot, the antibody specifically reacted with mGluR5 expressed in mammalian cells and rat brain. Immunohistochemical analysis revealed intense mGluR5-like immunoreactivity (LI) in the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, cerebral cortex, hippocampus, lateral septum, striatum, nucleus accumbens, inferior colliculus, and spinal trigeminal nuclei. The distribution pattern of mGluR5-LI corresponds very well with that of mGluR5 mRNA. Electron microscope analysis of the striatum revealed dense accumulation of immunoreaction products in dendrites which were often provided with asymmetrical synapses. These results suggest that mGluR5 is predominantly located in postsynaptic elements.

引用

页码：53 / 57

页数：5

共 19 条

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