GENE FUSION ANALYSIS OF MEMBRANE-PROTEIN TOPOLOGY - A DIRECT COMPARISON OF ALKALINE-PHOSPHATASE AND BETA-LACTAMASE FUSIONS

被引：28

作者：

PRINZ, WA ^{[1
]}

BECKWITH, J ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 20期

关键词：

D O I：

10.1128/jb.176.20.6410-6413.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to beta-lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent beta-lactamase fusions do not. This disparity may reflect differences in the folding of beta-lactamase and alkaline phosphatase in the cytoplasm.

引用

页码：6410 / 6413

页数：4

共 28 条

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