CHARACTERIZATION OF A BOUNDARY BETWEEN SATELLITE-III AND ALPHOID SEQUENCES ON HUMAN CHROMOSOME-10

被引：27

作者：

JACKSON, MS

MOLE, SE

PONDER, BAJ

机构：

[1] CRC Human Cancer Genetics Research Group, Department of Pathology, University of Cambridge, Cambridge CB2 lOP, Tennis Court Road

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 18期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/20.18.4781

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alphoid and satellite III sequences are arranged as large tandem arrays in the centromeric regions of human chromosomes. Several recent studies using in situ hybridisation to investigate the relative positions of these sequences have shown that they occupy adjacent but non-overlapping domains in metaphase chromosomes. We have analysed the DNA sequence at the junction between alphoid and satellite III sequences in a cosmid previously mapped to chromosome 10. The alphoid sequence consists of tandemly arranged dimers which are distinct from the known chromosome 10-specific alphoid family. Polymerase chain reaction experiments confirm the integrity of the sequence data. These results, together with pulsed field gel electrophoresis data place the boundary between alphoid and satellite III sequences in the mapping interval 10 centromere-10q11.2. The sequence data shows that these repetitive sequences are separated by a partial L1 interspersed repeat sequence less than 500bp in length. The arrangement of the junction suggests that a recombination event has brought these sequences into close proximity.

引用

页码：4781 / 4787

页数：7

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