ENGINEERING A UNIQUELY REACTIVE THIOL INTO A CYSTEINE-RICH PEPTIDE

被引：23

作者：

SHIMONY, E

SUN, TY

KOLMAKOVAPARTENSKY, L

MILLER, C

机构：

[1] Howard Hughes Medical Institute, Graduate Department of Biochemistry, Brandeis University, Waltham, MA

来源：

PROTEIN ENGINEERING | 1994年 / 7卷 / 04期

关键词：

CHARYBDOTOXIN; LABELING; POTASSIUM CHANNEL;

D O I：

10.1093/protein/7.4.503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cysteine mutagenesis for the purpose of chemical labelling was applied to the K+ channel neurotoxin charybdotoxin, a 37-residue peptide with six functionally essential cysteines. An additional 'spinster cysteine' was introduced at a position far away in space from the toxin's known interaction surface where it contacts its K+ channel receptor. Despite the presence of the extra unpaired cysteine residue, the toxin still folds efficiently and may be labelled by fluorescent and radioactive reagents to give a functionally competent toxin.

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页码：503 / 507

页数：5

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