DETERMINATION OF BRANCHED-CHAIN L-AMINO-ACIDS BY FLOW-INJECTION ANALYSIS WITH CO-IMMOBILIZED LEUCINE DEHYDROGENASE NADH OXIDASE AND CHEMILUMINESCENCE DETECTION

被引：11

作者：

KIBA, N

KATO, A

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 311卷 / 01期

关键词：

CHEMILUMINESCENCE; FLOW INJECTION; ENZYMATIC METHODS; AMINO ACIDS; PLASMA;

D O I：

10.1016/0003-2670(95)00146-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of branched-chain L-amino acids in plasma is described. Leucine dehydrogenase and NADH oxidase were co-immobilized on aminated poly(vinyl alcohol) beads and incorporated in a now-injection system with chemiluminescence detection. 20 mu l sample solution was injected into the system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(III) reaction. The calibration graph was linear for 5 X 10(-7)-6 X 10(-4) M; the detection limit (signal-to-noise ratio = 3) was 3 X 10(-7) M and the sample throughput was 15 h(-1) without carryover.

引用

收藏

页码：71 / 76

页数：6

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