CYCLOTHIALIDINE, A NOVEL DNA GYRASE INHIBITOR .2. ISOLATION, CHARACTERIZATION AND STRUCTURE ELUCIDATION

被引：23

作者：

KAMIYAMA, T

SHIMMA, N

OHTSUKA, T

NAKAYAMA, N

ITEZONO, Y

NAKADA, N

WATANABE, J

YOKOSE, K

机构：

[1] Nippon Roche Research Center, Kamakura, Kanagawa 247, Kajiwara

来源：

JOURNAL OF ANTIBIOTICS | 1994年 / 47卷 / 01期

关键词：

D O I：

10.7164/antibiotics.47.37

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cyclothialidine is a novel DNA gyrase inhibitor produced by Streptomyces filipinensis NR 0484. It was isolated from the culture broth by charcoal adsorption, Diaion HP-21, Amberlite CG-50, DEAE Toyopearl, and Toyopearl HW-40 SF column chromatography. The structure of cyclothialidine was determined to be a unique twelve membered lactone by amino acid analysis and various 2D-NMR experiments. Cyclothialidine inhibited Escherichia coli DNA gyrase with an IC50 of 30 ng/ml.

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页码：37 / 45

页数：9

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