GYRB MUTATIONS IN COUMERMYCIN A(1)-RESISTANT BORRELIA-BURGDORFERI

被引：57

作者：

SAMUELS, DS ^{[1
]}

MARCONI, RT ^{[1
]}

HUANG, WM ^{[1
]}

GARON, CF ^{[1
]}

机构：

[1] UNIV UTAH,MED CTR,DEPT CELLULAR VIRAL & MOLEC BIOL,SALT LAKE CITY,UT 84132

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 10期

关键词：

D O I：

10.1128/JB.176.10.3072-3075.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycinn A(1) which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A(1) than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

引用

页码：3072 / 3075

页数：4

共 41 条

[1] THE 43-KILODALTON N-TERMINAL FRAGMENT OF THE DNA GYRASE-B PROTEIN HYDROLYZES ATP AND BINDS COUMARIN DRUGS [J].

ALI, JA ;

JACKSON, AP ;

HOWELLS, AJ ;

MAXWELL, A .

BIOCHEMISTRY, 1993, 32 (10) :2717-2724

[2] LINEAR PLASMIDS OF THE BACTERIUM BORRELIA-BURGDORFERI HAVE COVALENTLY CLOSED ENDS [J].

BARBOUR, AG ;

GARON, CF .

SCIENCE, 1987, 237 (4813) :409-411

[3] LINEAR CHROMOSOME OF BORRELIA-BURGDORFERI [J].

BARIL, C ;

RICHAUD, C ;

BARANTON, G ;

STGIRONS, I .

RESEARCH IN MICROBIOLOGY, 1989, 140 (08) :507-516

[4] LYME-DISEASE - A TICK-BORNE SPIROCHETOSIS [J].

BURGDORFER, W ;

BARBOUR, AG ;

HAYES, SF ;

BENACH, JL ;

GRUNWALDT, E ;

DAVIS, JP .

SCIENCE, 1982, 216 (4552) :1317-1319

[5] LINEAR CHROMOSOMAL PHYSICAL AND GENETIC-MAP OF BORRELIA-BURGDORFERI, THE LYME-DISEASE AGENT [J].

CASJENS, S ;

HUANG, WM .

MOLECULAR MICROBIOLOGY, 1993, 8 (05) :967-980

[6]

CINCO M, 1992, FEMS MICROBIOL LETT, V92, P15, DOI 10.1111/j.1574-6968.1992.tb05227.x

[7] SELECTION OF AN ESCAPE VARIANT OF BORRELIA-BURGDORFERI BY USE OF BACTERICIDAL MONOCLONAL-ANTIBODIES TO OSPB [J].

COLEMAN, JL ;

ROGERS, RC ;

BENACH, JL .

INFECTION AND IMMUNITY, 1992, 60 (08) :3098-3104

[8]

CONTRERAS A, 1992, MOL MICROBIOL, V6, P1617

[9] DNA GYRASE AND THE SUPERCOILING OF DNA [J].

COZZARELLI, NR .

SCIENCE, 1980, 207 (4434) :953-960

[10] PHYSICAL MAP OF THE LINEAR CHROMOSOME OF THE BACTERIUM BORRELIA-BURGDORFERI-212, A CAUSATIVE AGENT OF LYME-DISEASE, AND LOCALIZATION OF RIBOSOMAL-RNA GENES [J].

DAVIDSON, BE ;

MACDOUGALL, J ;

GIRONS, IS .

JOURNAL OF BACTERIOLOGY, 1992, 174 (11) :3766-3774

← 1 2 3 4 5 →