MASSIVE CLUSTER IMPACT MASS-SPECTROMETRY - A NEW DESORPTION METHOD FOR THE ANALYSIS OF LARGE BIOMOLECULES

被引：94

作者：

MAHONEY, JF ^{[1
]}

PEREL, J ^{[1
]}

RUATTA, SA ^{[1
]}

MARTINO, PA ^{[1
]}

HUSAIN, S ^{[1
]}

LEE, TD ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL,DUARTE,CA 91010

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1991年 / 5卷 / 10期

关键词：

D O I：

10.1002/rcm.1290051004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.

引用

页码：441 / 445

页数：5

共 37 条

[1] SECONDARY ION MASS-SPECTROMETRY WITH CESIUM ION PRIMARY BEAM AND LIQUID TARGET MATRIX FOR ANALYSIS OF BIOORGANIC COMPOUNDS [J].

ABERTH, W ;

STRAUB, KM ;

BURLINGAME, AL .

ANALYTICAL CHEMISTRY, 1982, 54 (12) :2029-2034

[2]

AHRENS TJ, 1985, ICES SOLAR SYSTEM

[3] COMPARISON OF POLYATOMIC AND ATOMIC PRIMARY BEAMS FOR SECONDARY ION MASS-SPECTROMETRY OF ORGANICS [J].

APPELHANS, AD ;

DELMORE, JE .

ANALYTICAL CHEMISTRY, 1989, 61 (10) :1087-1093

[4] FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY [J].

BARBER, M ;

BORDOLI, RS ;

SEDGWICK, RD ;

TYLER, AN .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) :325-327

[5] RAPID HEATING IN FRAGILE MOLECULE MASS-SPECTROMETRY [J].

BEUHLER, RJ ;

FRIEDMAN, L .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 78 :1-15

[6] MASS DISTRIBUTIONS OF IONS SPUTTERED BY CLUSTER IMPACTS ON CARBON, COPPER AND GOLD TARGETS [J].

BEUHLER, RJ ;

FRIEDMAN, L .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1989, 94 (1-2) :25-39

[7] SHOCK-WAVE MECHANISM FOR CLUSTER EMISSION AND ORGANIC-MOLECULE DESORPTION UNDER HEAVY-ION BOMBARDMENT [J].

BITENSKY, IS ;

PARILIS, ES .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1987, 21 (01) :26-36

[8] SHOCK-WAVE MECHANISM FOR BIOMOLECULE DESORPTION UNDER HEAVY-ION BOMBARDMENT [J].

BITENSKY, IS ;

GOLDENBERG, AM ;

PARILIS, ES .

JOURNAL DE PHYSIQUE, 1989, 50 (C-2) :213-225

[9] SECONDARY-ION YIELDS FROM SURFACES BOMBARDED WITH KEV MOLECULAR AND CLUSTER IONS [J].

BLAIN, MG ;

DELLANEGRA, S ;

JORET, H ;

LEBEYEC, Y ;

SCHWEIKERT, EA .

PHYSICAL REVIEW LETTERS, 1989, 63 (15) :1625-1628

[10] CLUSTERS AS PROJECTILES FOR SIMS [J].

BLAIN, MG ;

SCHWEIKERT, EA ;

DASILVEIRA, EF .

JOURNAL DE PHYSIQUE, 1989, 50 (C-2) :85-92

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