MASSIVE CLUSTER IMPACT MASS-SPECTROMETRY - A NEW DESORPTION METHOD FOR THE ANALYSIS OF LARGE BIOMOLECULES

被引：94

作者：

MAHONEY, JF ^{[1
]}

PEREL, J ^{[1
]}

RUATTA, SA ^{[1
]}

MARTINO, PA ^{[1
]}

HUSAIN, S ^{[1
]}

LEE, TD ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL,DUARTE,CA 91010

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1991年 / 5卷 / 10期

关键词：

D O I：

10.1002/rcm.1290051004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.

引用

页码：441 / 445

页数：5

共 37 条

[11] SHOCK-LIKE PROCESSES IN ION SOLID INTERACTIONS [J].

CARTER, G .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH, 1983, 209 (MAY) :1-11

[12] SPIKE AND SHOCK PROCESSES IN HIGH-ENERGY DEPOSITION DENSITY ATOMIC COLLISION EVENTS IN SOLIDS [J].

CARTER, G .

RADIATION EFFECTS LETTERS, 1979, 43 (4-5) :193-199

[13] ELECTROHYDRODYNAMIC MASS-SPECTROMETRY [J].

COOK, KD .

MASS SPECTROMETRY REVIEWS, 1986, 5 (04) :467-519

[14] EFFECTS OF PRIMARY ION POLYATOMICITY AND KINETIC-ENERGY ON SECONDARY ION YIELD AND INTERNAL ENERGY IN SIMS [J].

HAND, OW ;

MAJUMDAR, TK ;

COOKS, RG .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1990, 97 (01) :35-45

[15] NON-LINEAR EFFECTS OF MOLECULAR ION-BOMBARDMENT ON SPUTTERING YIELD [J].

KARASHIMA, S .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS, 1982, 21 (03) :434-439

[16]

KATAZOE Y, 1980, RADIAT EFF LETT, V50, P39

[17]

Kinslow R., 1970, HIGH VELOCITY IMPACT

[18] MODELS FOR HIGH-ENERGY ION INDUCED DESORPTION OF MOLECULES FROM SURFACES [J].

LEE, SL ;

LUCCHESE, RR .

JOURNAL DE PHYSIQUE, 1989, 50 (C-2) :231-236

[19] MECHANISM FOR HIGH-MASS SAMPLE ION DESOLVATION IN ELECTROHYDRODYNAMIC MASS-SPECTROMETRY [J].

MAHONEY, JF ;

PEREL, J ;

LEE, TD ;

LEGESSE, K .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 79 (03) :249-266

[20] LARGE, ENERGETIC CLUSTER IMPACTS ON SURFACES [J].

MATTHEW, MW ;

BEUHLER, RJ ;

LEDBETTER, M ;

FRIEDMAN, L .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1986, 14 (4-6) :448-460

← 1 2 3 4 →